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Measuring the Kinase Activities of the LATS/NDR Protein Kinases

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

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Abstract

The Hippo tissue growth control and regeneration pathway is a main regulator of the YAP/TAZ effectors. In this regard, the LATS/NDR serine/threonine protein kinases can function as central components of the Hippo core module. More specifically, LATS/NDR-mediated phosphorylation of YAP/TAZ on different residues can regulate the subcellular localization and/or stability of YAP/TAZ. Therefore, the assessment of LATS/NDR activities can serve as readout for the activity status of the Hippo pathway. Here, we describe our preferred methodology regarding the measurement of the activities of LATS/NDR kinases.

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Acknowledgments

The Hergovich laboratory was supported by the Wellcome Trust (090090/Z/09/Z), BBSRC (BB/I021248/1), Worldwide Cancer Research (AICR; 11-0634), UCL Cancer Research UK Centre, and the National Institute for Health Research University College London Hospitals Biomedical Research Centre.

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  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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  1. Alexander Hergovich
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Correspondence to Alexander Hergovich .

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  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Hergovich, A. (2019). Measuring the Kinase Activities of the LATS/NDR Protein Kinases. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

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