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Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE

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Book cover Systems Chemical Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1888))

Abstract

Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.

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Acknowledgments

We thank Ms. H. Kondo, Ms. K. Noda, Ms. Y. Nakata, Ms. Y. Hirata, and Ms. M. Tanaka for conducting proteomic analysis. This work was supported in part by JSPS KAKENHI Grant Numbers JP16H06276, JP17H06412, JP18H03945, JP17K07783, AMED under Grant Number JP18cm0106112 and the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology).

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Authors and Affiliations

  1. Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan

    Makoto Muroi & Hiroyuki Osada

Authors
  1. Makoto Muroi
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  2. Hiroyuki Osada
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Corresponding author

Correspondence to Hiroyuki Osada .

Editor information

Editors and Affiliations

  1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Slava Ziegler

  2. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Herbert Waldmann

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Muroi, M., Osada, H. (2019). Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE. In: Ziegler, S., Waldmann, H. (eds) Systems Chemical Biology. Methods in Molecular Biology, vol 1888. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8891-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8891-4_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8890-7

  • Online ISBN: 978-1-4939-8891-4

  • eBook Packages: Springer Protocols

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