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BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

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Book cover BCL-2 Family Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1877))

Abstract

Biomolecular nuclear magnetic resonance (NMR) is a powerful and versatile method for studying both protein–protein interactions (PPIs) and protein–small molecule binding. NMR has been used extensively in the investigation of BCL-2 family proteins revealing the structure of key family members, identifying binding partners and interaction sites, and screening small molecule modulators. In this chapter we discuss the application of NMR to identify interaction sites and structure determination of protein–protein and protein–small molecule complexes using two examples.

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Acknowledgments

We would like to thank current and past members of the Gavathiotis Laboratory for contributing to the optimization of this protocol and research. This work was supported by an NCI grant 1R01CA178394 and awards from the Sidney Kimmel Foundation for Cancer Research, the Gabrielle’s Angels Foundation for Cancer Research, the Alexandrine and Alexander L. Sinsheimer Foundation, the Pershing Square Sohn Cancer Research Alliance, the American Heart Association Collaborative Science Award (15CSA26240000), the Fondation Leducq Transatlantic Network of Excellence grant (RA15CVD04) and the Irma T. Hirschl Trust Career Award. NMR data were collected with support from NIH awards 1S10OD016305 and P30 CA013330.

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Authors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA

    Thomas P. Garner & Evripidis Gavathiotis

  2. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA

    Thomas P. Garner & Evripidis Gavathiotis

  3. Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY, USA

    Thomas P. Garner & Evripidis Gavathiotis

  4. Wilf Family Cardiovascular Research Institute, Albert Einstein College of Medicine, Bronx, NY, USA

    Thomas P. Garner & Evripidis Gavathiotis

Authors
  1. Thomas P. Garner
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  2. Evripidis Gavathiotis
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Correspondence to Evripidis Gavathiotis .

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Editors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA

    Evripidis Gavathiotis

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Garner, T.P., Gavathiotis, E. (2019). BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy. In: Gavathiotis, E. (eds) BCL-2 Family Proteins. Methods in Molecular Biology, vol 1877. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8861-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8861-7_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8860-0

  • Online ISBN: 978-1-4939-8861-7

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