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Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level

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Book cover Functional Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1871))

Abstract

Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.

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Acknowledgments

This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601) and TE02000177 (TACR), and by Brno PhD Talent 2017 (funded by Brno City Municipality) and IGA grant no. IP 15/2017 to H.H.

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Authors and Affiliations

  1. Faculty of AgriSciences, Department of Molecular Biology and Radiobiology, CEITEC – Central European Institute of Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic

    Martin Černý, Miroslav Berka & Hana Habánová

Authors
  1. Martin Černý
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  2. Miroslav Berka
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  3. Hana Habánová
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Editor information

Editors and Affiliations

  1. Array Bridge Inc., St. Louis, MO, USA

    Xing Wang

  2. Biotech Support Group LLC, Monmouth Junction, NJ, USA

    Matthew Kuruc

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Černý, M., Berka, M., Habánová, H. (2019). Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level. In: Wang, X., Kuruc, M. (eds) Functional Proteomics. Methods in Molecular Biology, vol 1871. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8814-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8814-3_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8813-6

  • Online ISBN: 978-1-4939-8814-3

  • eBook Packages: Springer Protocols

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