Abstract
The rapid growing of genome editing technology leads to an optimistic expectation for the treatment of many incurable diseases. The core of genome editing relies on DNA-repairing processes which occur independently in each cell, thus generating a mix of genetic variation cells. Here, we describe the protocol of using mesenchymal stem cells as a platform to generate high purity of ZFN-edited patients’ cell clones which may be useful in conjunction with therapeutic cell conversion and reprograming.
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Manotham, K., Chattong, S. (2018). Genome Editing of MSCs as a Platform for Cell Therapy. In: Liu, J. (eds) Zinc Finger Proteins. Methods in Molecular Biology, vol 1867. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8799-3_10
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DOI: https://doi.org/10.1007/978-1-4939-8799-3_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8798-6
Online ISBN: 978-1-4939-8799-3
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