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Morphogen Gradients pp 3–17Cite as

Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1863))

Abstract

The regulatory mechanisms involved in plant development include many signals, some of them acting as graded positional cues regulating gene expression in a concentration-dependent manner. These regulatory molecules, that can be considered similar to animal morphogens, control cell behavior in developing organs. A suitable experimental approach to study expression gradients in plants is quantitative laser scanning confocal microscopy (LSCM) using Arabidopsis thaliana root tips as a model system. In this chapter, we outline a detailed method for image acquisition using LSCM, including detailed microscope settings and image analysis using FIJI as software platform.

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Change history

  • 07 December 2018

    The book was published with the following errors: In Chapter 1, figure 1 was stretched. This misconfiguration has now been modified.

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Acknowledgments

We thank Philip Benfey for the AGL42-GFP reporter, Moritz Nowack for the pSMB-H2B-GFP reporter, Lieven de Veylder for the pGL2:H2B-YFP, pCO:H2B-YFP, and the pEND:H2B-YFP reporters, and, finally, Ben Scheres for the PLT1 and PLT2 transcriptional and translational reporters. We thank Carla Schommer for critical reading of the manuscript. MFE and CG were supported by fellowships from CONICET. RV is a technician of CONICET. JP and RER are members of CONICET and are supported by grants from ANPCyT.

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Authors and Affiliations

  1. IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET, Universidad Nacional de Rosario, Rosario, Argentina

    María Florencia Ercoli, Rodrigo Vena, Camila Goldy, Javier F. Palatnik & Ramiro E. Rodríguez

  2. Centro de Estudios Interdisciplinarios, Universidad Nacional de Rosario, Rosario, Argentina

    Javier F. Palatnik & Ramiro E. Rodríguez

Authors
  1. María Florencia Ercoli
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  2. Rodrigo Vena
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  3. Camila Goldy
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  4. Javier F. Palatnik
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  5. Ramiro E. Rodríguez
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Corresponding author

Correspondence to Ramiro E. Rodríguez .

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Editors and Affiliations

  1. Integrated Microscopy Core, Baylor College of Medicine, Houston, TX, USA

    Julien Dubrulle

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Ercoli, M.F., Vena, R., Goldy, C., Palatnik, J.F., Rodríguez, R.E. (2018). Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots. In: Dubrulle, J. (eds) Morphogen Gradients. Methods in Molecular Biology, vol 1863. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8772-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8772-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8771-9

  • Online ISBN: 978-1-4939-8772-6

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