Abstract
Metabolic alterations are a hallmark of cancer. While determining metabolic changes in vitro has delivered valuable insight into the metabolism of cancer cells, it emerges that determining the in vivo metabolism adds an additional layer of information. Here, we therefore describe how to measure the in vivo metabolism of cancer tissue using 13C glucose infusions in mice.
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Acknowledgments
We thank Juan Fernández-GarcÃa for his feedback on the protocol. S.-M.F. acknowledges funding from the European Research Council under the ERC Consolidator Grant Agreement n. 771486–MetaRegulation; Marie Curie—CIG, FWO—Odysseus II, FWO—Research Grants/Projects, Eugène Yourassowsky Schenking, and KU Leuven—Methusalem Co-Funding. We would like to acknowledge http://www.somersault1824.com for image elements used in Fig. 6 (Creative Commons license CC BY-NC-SA 4.0).
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Broekaert, D., Fendt, SM. (2019). Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_5
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DOI: https://doi.org/10.1007/978-1-4939-8769-6_5
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