Abstract
Live imaging gives additional layers of information such as physical dynamics of a molecule of your interest. Aptamer-based green fluorescent protein (GFP) labeling is suitable for visualization of RNA molecules. Here we describe a method to visualize Xist RNA using the Bgl aptamer system.
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Acknowledgments
I thank Drs. Edith Heard and Haruhiko Koseki for critical discussions. This work was supported by PRESTO and KAKENHI to O.M.
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Masui, O., Heard, E., Koseki, H. (2018). Live Imaging of Xist RNA. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_6
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DOI: https://doi.org/10.1007/978-1-4939-8766-5_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-8765-8
Online ISBN: 978-1-4939-8766-5
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