Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain

  • Protocol
  • First Online:
Protein Gel Detection and Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

Abstract

Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic-acid Schiff’s base which uses acidic fuchsin dye.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Spiro R (2002) Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology 12:43R–56R

    Article  CAS  PubMed  Google Scholar 

  2. Wandall HH, Blixt O, Tarp MA, Pedersen JW, Bennett EP, Mandel U, Ragupathi G, Livingston PO, Hollingsworth MA, Taylor-Papadimitriou J, Burchell J, Clausen H (2010) Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopes. Cancer Res 70:1306–1313

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Molecular Probes: Product Information (2007)

    Google Scholar 

  4. McEver RP (2002) Selectins: lectins that initiate cell adhesion under flow. Curr Opin Cell Biol 14:581–586

    Article  CAS  PubMed  Google Scholar 

  5. Stearns DJ, Kurosawa S, Sims PJ, Esmon NL, Esmon CT (1988) The interaction of a Ca2+-dependent monoclonal antibody with the protein C activation peptide region. Evidence for obligatory Ca2+ binding to both antigen and antibody. J Biol Chem 263:826–832

    PubMed  CAS  Google Scholar 

  6. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The author thanks Dr. Charles Esmon for the HPC4 antibody and Dr. Rodger P. McEver for purified soluble PSGL-1.

Author information

Authors and Affiliations

  1. Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA

    Padmaja Mehta-D’souza

Authors
  1. Padmaja Mehta-D’souza
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Padmaja Mehta-D’souza .

Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Mehta-D’souza, P. (2018). Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation