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Fluorescent Staining of Gels

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Protein Gel Detection and Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

Abstract

Certain transition metal complexes show intensive fluorescence when bound to proteins. They can be used to stain gels after electrophoresis with a sensitivity approaching that of silver staining, but in a much simpler and more reproducible procedure. Stains can be prepared easily and at a fraction of the cost of commercially available reagents. Hydrophobic dyes can be used to stain gels without fixing; they do not interfere with later blotting or electroelution.

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Acknowledgments

This work was supported by funding from Ross University.

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Authors and Affiliations

  1. Kevelaer, Germany

    Engelbert Buxbaum

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  1. Engelbert Buxbaum
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Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Buxbaum, E. (2018). Fluorescent Staining of Gels. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

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