Skip to main content
SpringerLink
Log in

Isolating and Characterizing Adipose-Derived Stem Cells

  • Protocol
  • First Online:
Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1842))

Abstract

The research and application of regeneration medicine will require a reliable source of stem cells. Adipose tissue has proven to be an easily accessible and rich source of adult stem cells, termed adipose-derived stem cells (ASCs). ASCs have the most important advantage over stem cells from other available sources. There is no other human tissue as abundant as adipose tissue, making it possible to isolate adequate numbers of ASCs for potential clinical applications. Here, we describe detailed methods for isolating and characterizing ASCs. These procedures can be applied to adipose tissue not only for humans but also for other species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Zuk PA (2010) The adipose-derived stem cell: looking back and looking ahead. Mol Biol Cell 21(11):1783–1787

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gimble JM, Nuttall ME (2011) Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des 17(4):332–339

    Article  CAS  PubMed  Google Scholar 

  3. Gimble JM, Bunnell BA, Guilak F (2012) Human adipose-derived cells: an update on the transition to clinical translation. Regen Med 7(2):225–235

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, Campbell AM, Boucher S, Vemuri MC, Silva CL, Cabral JM (2011) Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions. Tissue Eng Part C Methods 17(12):1201–1210

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Mizuno H, Tobita M, Uysal AC (2012) Adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells 30(5):804–810

    Article  CAS  PubMed  Google Scholar 

  6. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Dj P, Horwitz E (2006) Cytotherapy 8(4):315–317

    Article  CAS  PubMed  Google Scholar 

  7. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C (2008) Adipose-derived stem cells: isolation, expansion and differentiation. Methods 45(2):115–120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Kretlow JD, Jin YQ, Liu W, Zhang WJ, Hong TH, Zhou G, Baggett LS, Mikos AG, Cao Y (2008) Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol 9:60

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Natural Science Foundation of China (Research number 31271027 and 81171475).

Author information

Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Shanghai Tenth People’s Hospital, TongJi University School of Medicine, Shanghai, People’s Republic of China

    Guangpeng Liu & Xi Chen

Authors
  1. Guangpeng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, USA

    Pranela Rameshwar

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Liu, G., Chen, X. (2018). Isolating and Characterizing Adipose-Derived Stem Cells. In: Singh, S., Rameshwar, P. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 1842. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8697-2_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8697-2_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8696-5

  • Online ISBN: 978-1-4939-8697-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation