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Proteomic Signatures in Staphylococcus aureus

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Microbial Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1841))

Abstract

Despite all its apparent limitations proteome analysis based on two-dimensional protein gels combined with mass spectrometry is still the method of choice to study global protein synthesis activity in bacterial cells. Alterations in global protein synthesis play an important role during adaptation of bacteria to changing environmental conditions which are rather the role than the exception in their natural habitats. The protein synthesis pattern in response to a certain stimulus is highly specific and reflects the new challenges the bacterium has to meet. Here we present the techniques to analyze global protein synthesis in bacteria as exemplified by Staphylococcus aureus which is an important human pathogen and one main cause of nosocomial infections with severe outcome.

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Acknowledgments

We are very grateful to our former colleagues at the Institute for Microbiology of the Greifswald University who were involved in these experiments. Furthermore, we thank the Decodon GmbH (Greifswald) for providing the Delta2D software. This work was supported by grants from the Bildungsministerium für Bildung und Forschung (InfektionsGenomik) and the Deutsche Forschungsgemeinschaft (SFB-TRR34, FOR 585, GRK840).

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Authors and Affiliations

  1. Institute for Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Susanne Engelmann

  2. Microbial Proteomics, Helmholtz Centre for Infection Research, Braunschweig, Germany

    Susanne Engelmann

  3. Robert Koch Institute, Wernigerode, Germany

    Stephan Fuchs

Authors
  1. Susanne Engelmann
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  2. Stephan Fuchs
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Corresponding author

Correspondence to Susanne Engelmann .

Editor information

Editors and Affiliations

  1. Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany

    Dörte Becher

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Engelmann, S., Fuchs, S. (2018). Proteomic Signatures in Staphylococcus aureus. In: Becher, D. (eds) Microbial Proteomics. Methods in Molecular Biology, vol 1841. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8695-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8695-8_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8693-4

  • Online ISBN: 978-1-4939-8695-8

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