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Microbial Proteomics pp 207–228Cite as

In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1841))

Abstract

Proteome profiling of bacteria internalized by host cells is still a challenging task, due to low amounts of bacterial proteins in host–pathogen settings and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 106 internalized bacterial cells as starting material.

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Acknowledgments

This work was supported by the German Research Foundation Grant SFB/TR34.

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Authors and Affiliations

  1. Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany

    Henrike Pförtner, Maren Depke & Kristin Surmann

  2. Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany

    Frank Schmidt & Uwe Völker

Authors
  1. Henrike Pförtner
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  2. Maren Depke
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  3. Kristin Surmann
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  4. Frank Schmidt
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  5. Uwe Völker
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Corresponding author

Correspondence to Uwe Völker .

Editor information

Editors and Affiliations

  1. Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany

    Dörte Becher

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Pförtner, H., Depke, M., Surmann, K., Schmidt, F., Völker, U. (2018). In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings. In: Becher, D. (eds) Microbial Proteomics. Methods in Molecular Biology, vol 1841. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8695-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8695-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8693-4

  • Online ISBN: 978-1-4939-8695-8

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