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Identifying Target RNAs of PARPs

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Book cover ADP-ribosylation and NAD+ Utilizing Enzymes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1813))

Abstract

Posttranscriptional regulation of RNA is an important component of gene expression by controlling the total amount of mRNA available for translation into protein. It involves multiple pathways including nuclear processing of mRNA and its precursors, RNA silencing, and regulation of RNA decay. Poly(ADP-ribose) polymerases (PARPs), enzymes that modify target proteins with ADP-ribose, play important roles in several RNA-regulatory pathways. RNA-binding PARPs target specific transcripts for regulation, and multiple PARPs ADP-ribosylate RNA-regulatory proteins to alter their localization, activity, or RNA binding. Additionally, RNA-binding proteins can bind directly to poly(ADP-ribose) with various effects on their function. Here we describe methods to identify and confirm specific transcripts that are regulated by PARPs.

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Acknowledgments

This work was partially supported by Cancer Center Support (core; grant P30-CA14051) and RO1GM087465 from the National Institutes of Health to PC. FJB was funded by a Ludwig Postdoctoral Fellowship.

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Author notes

  1. Paul Chang

    Present address: Ribon Therapeutics, Lexington, MA, USA

Authors and Affiliations

  1. Cancer Research UK Beatson Institute, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK

    Florian J. Bock

  2. Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA

    Paul Chang

Authors
  1. Florian J. Bock
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  2. Paul Chang
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Editor information

Editors and Affiliations

  1. Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA

    Paul Chang

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Bock, F.J., Chang, P. (2018). Identifying Target RNAs of PARPs. In: Chang, P. (eds) ADP-ribosylation and NAD+ Utilizing Enzymes. Methods in Molecular Biology, vol 1813. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8588-3_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8588-3_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8587-6

  • Online ISBN: 978-1-4939-8588-3

  • eBook Packages: Springer Protocols

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