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Protein Scaffolds pp 213–221Cite as

Genipin Cross-Linking of Elastin and Elastin-Based Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1798))

Abstract

Genipin is a natural plant-derived compound that covalently cross-links biopolymers into lattice networks with good biocompatibility, controllable swelling, and mechanical properties. This protocol describes the genipin cross-linking of elastic proteins, including tropoelastin and elastin-based polypeptides, through steps of elastin phase-separation upon addition of salt and heat, centrifugation to rapidly concentrate the dense protein phase, and incubation. This method is applicable for the fabrication of elastic materials suitable for use as scaffolds for biomedical applications.

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Acknowledgments

The author thanks Fred Keeley and Sean Reichheld for useful discussions.

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Authors and Affiliations

  1. Molecular Medicine Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada

    Lisa D. Muiznieks

Authors
  1. Lisa D. Muiznieks
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Editors and Affiliations

  1. Department of Chemistry, Occidental College, Los Angeles, California, USA

    Andrew K. Udit

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Muiznieks, L.D. (2018). Genipin Cross-Linking of Elastin and Elastin-Based Proteins. In: Udit, A. (eds) Protein Scaffolds. Methods in Molecular Biology, vol 1798. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7893-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7893-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7892-2

  • Online ISBN: 978-1-4939-7893-9

  • eBook Packages: Springer Protocols

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