Abstract
Immunochemistry (immunocytochemistry for cells and immunohistochemistry for tissues) is a method used to label specific antigens, based on highly specific antibody–epitope interactions. The resulting labeling can be visualized and imaged through microscopy adapted to the type of detection system used (fluorophore, peroxidase, etc.). In the nervous system, myelin is a complex membrane structure, generated by myelinating glial cells, which ensheath axons and facilitate electrical conduction. Myelin alteration has been shown to occur in various neurological diseases, in which it is associated with functional deficits. Here, we focus on myelin detection by immunofluorescence using immunochemistry protocols based on antibodies directed against major myelin proteins.
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References
Desmazieres A, Sol-Foulon N, Lubetzki C (2012) Changes at the nodal and perinodal axonal domains: a basis for multiple sclerosis pathology? Mult Scler 18(2):133–137. https://doi.org/10.1177/1352458511434370
Devaux JJ (2012) Antibodies to gliomedin cause peripheral demyelinating neuropathy and the dismantling of the nodes of Ranvier. Am J Pathol 181(4):1402–1413. https://doi.org/10.1016/j.ajpath.2012.06.034
Cammer W, Norton WT (1976) Disc gel electrophoresis of myelin proteins: new observations on development of the intermediate proteins (DM-20). Brain Res 109(3):643–648
Baumann N, Pham-Dinh D (2001) Biology of oligodendrocyte and myelin in the mammalian central nervous system. Physiol Rev 81(2):871–927
Kluver H, Barrera E (1953) A method for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol 12(4):400–403
Meier C (1976) Some observations on early myelination in the human spinal cord. Light and electron microscope study. Brain Res 104(1):21–32
Hori SH (1963) A simplified acid hematein test for phospholipids. Stain Technol 38:221–225
Gallyas F (1979) Silver staining of myelin by means of physical development. Neurol Res 1(2):203–209
Freeman SA, Desmazieres A, Simonnet J, Gatta M, Pfeiffer F, Aigrot MS, Rappeneau Q, Guerreiro S, Michel PP, Yanagawa Y, Barbin G, Brophy PJ, Fricker D, Lubetzki C, Sol-Foulon N (2015) Acceleration of conduction velocity linked to clustering of nodal components precedes myelination. Proc Natl Acad Sci U S A 112(3):E321–E328. https://doi.org/10.1073/pnas.1419099112
Stoppini L, Buchs PA, Muller D (1991) A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 37(2):173–182
Tepavcevic V, Kerninon C, Aigrot MS, Meppiel E, Mozafari S, Arnould-Laurent R, Ravassard P, Kennedy TE, Nait-Oumesmar B, Lubetzki C (2014) Early netrin-1 expression impairs central nervous system remyelination. Ann Neurol 76(2):252–268. https://doi.org/10.1002/ana.24201
Spassky N, Olivier C, Cobos I, LeBras B, Goujet-Zalc C, Martinez S, Zalc B, Thomas JL (2001) The early steps of oligodendrogenesis: insights from the study of the plp lineage in the brain of chicks and rodents. Dev Neurosci 23(4–5):318–326
Liu J, Dietz K, DeLoyht JM, Pedre X, Kelkar D, Kaur J, Vialou V, Lobo MK, Dietz DM, Nestler EJ, Dupree J, Casaccia P (2012) Impaired adult myelination in the prefrontal cortex of socially isolated mice. Nat Neurosci 15(12):1621–1623. https://doi.org/10.1038/nn.3263
Yang HJ, Vainshtein A, Maik-Rachline G, Peles E (2016) G protein-coupled receptor 37 is a negative regulator of oligodendrocyte differentiation and myelination. Nat Commun 7:10884. https://doi.org/10.1038/ncomms10884
Giera S, Deng Y, Luo R, Ackerman SD, Mogha A, Monk KR, Ying Y, Jeong SJ, Makinodan M, Bialas AR, Chang BS, Stevens B, Corfas G, Piao X (2015) The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development. Nat Commun 6:6121. https://doi.org/10.1038/ncomms7121
Miron VE, Boyd A, Zhao JW, Yuen TJ, Ruckh JM, Shadrach JL, van Wijngaarden P, Wagers AJ, Williams A, Franklin RJM, Ffrench-Constant C (2013) M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination. Nat Neurosci 16(9):1211–1218. https://doi.org/10.1038/nn.3469
Domenech-Estevez E, Baloui H, Meng X, Zhang Y, Deinhardt K, Dupree JL, Einheber S, Chrast R, Salzer JL (2016) Akt regulates axon wrapping and myelin sheath thickness in the PNS. J Neurosci 36(16):4506–4521. https://doi.org/10.1523/JNEUROSCI.3521-15.2016
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Thetiot, M., Freeman, S.A., Desmazières, A. (2018). Immunohistochemical Analysis of Myelin Structures. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_2
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_2
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