Abstract
Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) + complete Freund’s adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG35–55.
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Acknowledgments
The authors are grateful to grants SAF2011-23272 and SAF2014-56546-R (Ministerio de Economía y Competitividad y Fondo Europeo de Desarrollo Regional) to J.H.
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Giralt, M., Molinero, A., Hidalgo, J. (2018). Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35–55 in the Mouse. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_17
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_17
Publisher Name: Humana Press, New York, NY
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