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Myelin pp 207–224Cite as

Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1791))

Abstract

Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive—new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.

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Author information

Authors and Affiliations

  1. Singapore Institute for Neurotechnology, National University of Singapore, Singapore, Singapore

    Agata Blasiak, Sudip Nag & In Hong Yang

  2. Department of Electronics and Electrical Communication Engineering, Indian Institute of Technology, Kharagpur, India

    Sudip Nag

  3. Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD, USA

    In Hong Yang

Authors
  1. Agata Blasiak
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  2. Sudip Nag
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  3. In Hong Yang
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Corresponding author

Correspondence to In Hong Yang .

Editor information

Editors and Affiliations

  1. Nerve Disorders Laboratory, CIC bioGUNE, Derio, Vizcaya, Spain

    Ashwin Woodhoo

  2. Basque Foundation for Science, IKERBASQUE, Bilbao, Vizcaya, Spain

    Ashwin Woodhoo

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Blasiak, A., Nag, S., Hong Yang, I. (2018). Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7862-5_16

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7861-8

  • Online ISBN: 978-1-4939-7862-5

  • eBook Packages: Springer Protocols

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