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RNA Interference-Mediated Gene Silencing in Esophageal Adenocarcinoma

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Esophageal Adenocarcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1756))

Abstract

RNA interference (RNAi) is a normal physiological mechanism in which a short effector antisense RNA molecule regulates target gene expression. It is a powerful tool to silence a particular gene of interest in a sequence-specific manner and can be used to target against various molecular pathways in esophageal adenocarcinoma by designing RNAi targeting key pathogenic genes. RNAi-based therapeutics against esophageal adenocarcinoma can be developed using different strategies including inhibition of overexpressed oncogenes, blocking cell division by interfering cyclins and related genes or enhancing apoptosis by suppressing anti-apoptotic genes. In addition, RNAi against multidrug resistance genes or chemo-resistance targets may provide promising cancer therapeutic options. Here, we describe RNAi technology using MET, a proto-oncogene in esophageal adenocarcinoma cells, as a model target. Lentiviral particles expressing MET shRNA was used to silence MET genes. Then, Western blot analysis was performed to confirm MET knockdown.

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Authors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Farhadul Islam, Vinod Gopalan & Alfred K. Lam

  2. Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

    Farhadul Islam

Authors
  1. Farhadul Islam
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  2. Vinod Gopalan
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  3. Alfred K. Lam
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Corresponding author

Correspondence to Alfred K. Lam .

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Editors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Alfred K. Lam

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Islam, F., Gopalan, V., Lam, A.K. (2018). RNA Interference-Mediated Gene Silencing in Esophageal Adenocarcinoma. In: Lam, A. (eds) Esophageal Adenocarcinoma. Methods in Molecular Biology, vol 1756. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7734-5_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7734-5_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7733-8

  • Online ISBN: 978-1-4939-7734-5

  • eBook Packages: Springer Protocols

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