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Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusks

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Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1747))

Abstract

Electronic paramagnetic resonance (EPR) is an appropriate tool to identify free radicals formed in tissues under normal as well as stressful conditions. Since nitric oxide (NO) as a free radical has paramagnetic properties it can be detected by EPR. The use of spin traps highly improves the sensitivity allowing NO identification, detection and quantification at room temperature in vitro and in vivo conditions. NO production in animals is almost exclusively associated to an enzyme family known as Nitric Oxide Synthases (NOSs). The digestive glands of mollusks are a major target for oxidative disruption related to environmental stress. A simple EPR-methodology to asses both, the presence of NO and its rate of generation in tissues from different mollusk species, is reported here.

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Acknowledgments

This study was supported by grants from the UBA (20020130100383BA), ANPCyT (PICT 00845), and CONICET (PIP 00697). S.P. and P.M.G. are career investigators from CONICET.

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Authors and Affiliations

  1. Facultad de Farmacia y Bioquímica, Fisicoquímica, Universidad de Buenos Aires, Buenos Aires, Argentina

    Paula Mariela González & Susana Puntarulo

  2. Instituto de Bioquímica y Medicina Molecular (IBIMOL), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina

    Paula Mariela González & Susana Puntarulo

Authors
  1. Paula Mariela González
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  2. Susana Puntarulo
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Corresponding author

Correspondence to Susana Puntarulo .

Editor information

Editors and Affiliations

  1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel

  2. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Christian Lindermayr

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González, P.M., Puntarulo, S. (2018). Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusks. In: Mengel, A., Lindermayr, C. (eds) Nitric Oxide. Methods in Molecular Biology, vol 1747. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7695-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7695-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7694-2

  • Online ISBN: 978-1-4939-7695-9

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