Abstract
Metabolomics is the area of research, which strives to obtain complete metabolic fingerprints, to detect differences between them, and to provide hypothesis to explain those differences [1]. But obtaining complete metabolic fingerprints is not an easy task. Metabolite extraction is a key step during this process, and much research has been devoted to finding the best solvent mixture to extract as much metabolites as possible.
Here a procedure is described for analysis of both polar and apolar metabolites using a two-phase extraction system. D2O and CDCl3 are the solvents of choice, and their major advantage is that, for the identification of the compounds, standard databases can be used because D2O and CDCl3 are the solvents most commonly used for pure compound NMR spectra. The procedure enables the absolute quantification of components via the addition of suitable internal standards. The extracts are also suitable for further analysis with other systems like LC-MS or GC-MS.
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References
Schripsema J, Dagnino D (2015) Metabolomics. In: Hostettmann K, Stuppner H, Marston A, Chen S (eds) Handbook of chemical and biological plant analytical methods, 1st edn. Wiley, New York
Schripsema J (2010) Application of NMR in plant metabolomics: techniques, problems and prospects. Phytochem Anal 21:14–21
Deda O, Gika HG, Wilson IA, Theodoridis GA (2015) An overview of fecal preparation for global metabolic profiling. J Pharm Biomed Anal 113:137–150
Kim H-S, Park SJ, Hyun S-H et al (2011) Biochemical monitoring of black raspberry (Rubus coreanus Miquel) fruits according to maturation stage by 1H NMR using multiple solvent systems. Food Res Int 44:1977–1987
Heyman HM, Meyer JJM (2012) NMR-based metabolomics as a quality control tool for herbal products. S Afr J Bot 82:21–32
Kim HK, Verpoorte R (2010) Sample preparation for plant metabolomics. Phytochem Anal 21:4–13
Beltran A, Suarez M, Rodríguez MA et al (2012) Assessment of compatibility between extraction methods for NMR and LC/MS-based metabolomics. Anal Chem 84:5838−5844
Kim HK, Choi YH, Verpoorte R (2010) NMR-based metabolomic analysis of plants. Nat Protoc 5:536–549
Schripsema J (2008) Comprehensive analysis of polar and apolar constituents of butter and margarine by Nuclear Magnetic Resonance, reflecting quality and production processes. J Agric Food Chem 56:2547–2552
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Schripsema, J., Dagnino, D. (2018). Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR. In: Theodoridis, G., Gika, H., Wilson, I. (eds) Metabolic Profiling. Methods in Molecular Biology, vol 1738. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7643-0_13
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DOI: https://doi.org/10.1007/978-1-4939-7643-0_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7643-0
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