Abstract
Gavi is intended for use in a laboratory or clinic environment for the preparation and vitrification of oocytes, cleavage stage embryos and blastocysts. Gavi is designed to automate the equilibration steps in the vitrification process to minimize the variability that occurs during cryopreservation. This automated process reduces the potential for errors and ensures a standardized, repeatable procedure for vitrification in a controlled, closed-system environment.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kuwayama M (2007) Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 67:73–80
Roy TK, Bradley CK, Bowman MC et al (2014) Single-embryo transfer of vitrified-warmed blastocysts yields equivalent live-birth rates and improved neonatal outcomes compared with fresh transfers. Fertil Steril 101:1294–1301
Kato O, Kawasaki N, Bodri D et al (2012) Neonatal outcome and birth defects in 6623 singletons born following minimal ovarian stimulation and vitrified versus fresh single embryo transfer. Eur J Obstet Gynecol Reprod Biol 161:46–50
Takahashi K, Mukaida T, Goto T et al (2005) Perinatal outcome of blastocyst transfer with vitrification using cryoloop: a 4-year follow-up study. Fertil Steril 84:88–92
Gosden R (2011) Cryopreservation: a cold look at technology for fertility preservation. Fertil Steril 96:264–268
Alpha Scientists in Reproductive Medicine (2012) The Alpha consensus meeting on cryopreservation key performance indicators and benchmarks: proceedings of an expert meeting. Reprod Biomed Online 25:146–167
Kader AA, Choi A, Orief Y et al (2009) Factors affecting the outcome of human blastocyst vitrification. Reprod Biol Endocrinol 7:99
Roy TK, Brandi S, Bradley CK et al (2016) Automatic vitrification: development of the Gavi system. In: Tucker MJ, Liebermann J (eds) Vitrification in Assisted Reproduction, 2nd edn. CRC Press, Florida
Roy TK, Brandi S, Tappe NM et al (2014) Embryo vitrification using a novel semi-automated closed system yields in vitro outcomes equivalent to manual Cryotop method. Hum Reprod 29(11):2431–2438
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Electronic Supplementary Materials
Gavi vitrification (MP4 684289 kb)
Gavi warming (MP4 379301 kb)
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Roy, T.K., Brandi, S., Peura, T.T. (2017). Chapter 20 Gavi-Automated Vitrification Instrument. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_20
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6828-2_20
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6826-8
Online ISBN: 978-1-4939-6828-2
eBook Packages: Springer Protocols