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Cell Sheet Technology for Cardiac Tissue Engineering

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1181))

Abstract

In this chapter, we describe the methods for the fabrication and transfer/transplantation of 3D tissues by using cell sheet technology for cardiac tissue regeneration. A temperature-responsive culture surface can be fabricated by grafting a temperature-responsive polymer, poly(N-isopropylacrylamide), onto a polystyrene cell culture surface. Cells cultured confluently on such a culture surface can be recovered as an intact cell sheet, and functional three-dimensional (3D) tissues can then be easily fabricated by layering the recovered cell sheets without any scaffolds or complicated manipulation. Cardiac cell sheets, myoblast sheets, mesenchymal stem cell sheets, cardiac progenitor cell sheets, etc., which are prepared from temperature-responsive culture surfaces, can be easily transplanted onto heart tissues of animal models, and those cell sheet constructs enhance the cell transplant efficiency, resulting in the induction of effective therapy.

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Acknowledgements

This work was supported by grants from Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology “Cell Sheet Tissue Engineering Center (CSTEC)” from the Ministry of Education, Culture, Sports Science, and Technology (MEXT), Japan, and JSPS through the “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program),” initiated by the Council for Science and Technology Policy (CSTP).

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Authors and Affiliations

  1. Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan

    Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Masayuki Yamato & Teruo Okano

  2. Department of Mechanical Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Japan

    Kenjiro Tadakuma & Makoto Kaneko

Authors
  1. Yuji Haraguchi
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  2. Tatsuya Shimizu
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  3. Katsuhisa Matsuura
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  4. Hidekazu Sekine
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  5. Nobuyuki Tanaka
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  6. Kenjiro Tadakuma
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  7. Masayuki Yamato
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  8. Makoto Kaneko
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  9. Teruo Okano
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Corresponding author

Correspondence to Teruo Okano .

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Editors and Affiliations

  1. Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Ontario, Canada

    Milica Radisic

  2. Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA

    Lauren D. Black III

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Haraguchi, Y. et al. (2014). Cell Sheet Technology for Cardiac Tissue Engineering. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1047-2_13

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