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Virus-Induced Gene Silencing in Plants pp 183–197Cite as

ALSV-Based Virus-Induced Gene Silencing in Apple Tree (Malus × domestica L.)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2172))

Abstract

Virus-induced gene silencing (VIGS) is a fast and efficient tool to investigate gene function in plant as an alternative to knock down/out transgenic lines, especially in plant species difficult to transform and challenging to regenerate such as perennial woody plants. In apple tree, a VIGS vector has been previously developed based on the Apple latent spherical virus (ALSV) and an efficient inoculation method has been optimized using biolistics. This report described detailed step-by-step procedure to design and silence a gene of interest (GOI) in apple tree tissues using the ALSV-based vector.

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Author information

Authors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Carolina Werner Ribeiro, Thomas Dugé de Bernonville, Gaëlle Glévarec, Arnaud Lanoue, Audrey Oudin, Olivier Pichon, Benoit St-Pierre, Vincent Courdavault & Sébastien Besseau

Authors
  1. Carolina Werner Ribeiro
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  2. Thomas Dugé de Bernonville
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  3. Gaëlle Glévarec
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  4. Arnaud Lanoue
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  5. Audrey Oudin
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  6. Olivier Pichon
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  7. Benoit St-Pierre
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  8. Vincent Courdavault
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  9. Sébastien Besseau
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Corresponding author

Correspondence to Sébastien Besseau .

Editor information

Editors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sébastien Besseau

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Werner Ribeiro, C. et al. (2020). ALSV-Based Virus-Induced Gene Silencing in Apple Tree (Malus × domestica L.). In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0751-0_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0750-3

  • Online ISBN: 978-1-0716-0751-0

  • eBook Packages: Springer Protocols

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