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A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

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Kupffer Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2164))

Abstract

Transgenic mouse are reliable, convenient models for studying human hepatocellular carcinoma (HCC). The development of a synthetically engineered Sleeping Beauty (SB) transposon system further enables the viral-free, efficient delivery of desired oncogenes to mouse tissues. Here, we describe an SB transposon-based approach to induce HCC in mice by expressing a hyperactive form of N-RAS, N-RASG12V, while silencing the endogenous Trp53 gene via hydrodynamic tail vein injection, a method to rapidly deliver naked plasmids to mouse liver.

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Authors and Affiliations

  1. MRC London Institute of Medical Sciences, Hammersmith Hospital Campus, London, UK

    Sijia Yu & Santiago Vernia

  2. Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK

    Sijia Yu & Santiago Vernia

Authors
  1. Sijia Yu
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  2. Santiago Vernia
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Corresponding author

Correspondence to Santiago Vernia .

Editor information

Editors and Affiliations

  1. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Myriam Aouadi

  2. Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden

    Valerio Azzimato

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Yu, S., Vernia, S. (2020). A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0704-6_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0703-9

  • Online ISBN: 978-1-0716-0704-6

  • eBook Packages: Springer Protocols

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