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Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2156))

Abstract

Plasma membrane is the primary determinant of freezing tolerance in plants because of its central role in freeze–thaw cycle. Changes in plasma membrane protein composition have been one of the major research areas in plant cold acclimation. To obtain comprehensive profiles of the plasma membrane proteomes and their changes during the cold acclimation process, a plasma membrane purification method using a dextran–polyethylene glycol two polymer system and a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for the plasma membrane proteins are described. The proteomic results obtained are further applied to label-free protein semiquantification.

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Acknowledgments

This work was supported in part by a Research Fellowship for Young Scientists (#247373 to D.T.) and Grants-in-Aid for Scientific Research (#22120003, #24370018, #17H03961 to M.U. and Y.K.) from JSPS, Japan.

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Author notes

  1. Daisuke Takahashi

    Present address: Graduate School of Science & Engineering, Saitama University, Saitama, Japan

Authors and Affiliations

  1. United Graduate School of Agricultural Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

    Md Mostafa Kamal, Daisuke Takahashi, Yukio Kawamura & Matsuo Uemura

  2. Department of Plant-Bioscience, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

    Takato Nakayama, Yushi Miki, Yukio Kawamura & Matsuo Uemura

Authors
  1. Md Mostafa Kamal
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  2. Daisuke Takahashi
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  3. Takato Nakayama
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  4. Yushi Miki
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  5. Yukio Kawamura
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  6. Matsuo Uemura
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Corresponding author

Correspondence to Matsuo Uemura .

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Editors and Affiliations

  1. Max-Planck-Institut fĂĽr Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha

  2. Max-Planck-Institut fĂĽr Molekulare Pflanzenphysiologie, Potsdam, Germany

    Ellen Zuther

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Kamal, M.M., Takahashi, D., Nakayama, T., Miki, Y., Kawamura, Y., Uemura, M. (2020). Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 2156. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0660-5_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0660-5_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0659-9

  • Online ISBN: 978-1-0716-0660-5

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