Abstract
We describe an extrusion-based method to print a human bilayered skin using bioinks containing human plasma and primary human fibroblasts and keratinocytes from skin biopsies. We generate 100 cm2 of printed skin in less than 35 min. We analyze its structure using histological and immunohistochemical methods, both in in vitro 3D cultures and upon transplantation to immunodeficient mice. We have demonstrated that the printed skin is similar to normal human skin and indistinguishable from bilayered dermo-epidermal equivalents, previously produced manually in our laboratory and successfully used in the clinic.
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Acknowledgments
We kindly thank Prof. José Luis Jorcano (from UC3M) and Prof. Juan F. del Cañizo (Hospital General Universitario Gregorio Marañón) for their guidance and support. This work was supported by Programa de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM, Programa Estatal de I+D+i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00 and Cátedra Fundación Ramón Areces. The authors declare no conflict of interest.
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Quílez, C. et al. (2020). Bioprinting for Skin. In: Crook, J.M. (eds) 3D Bioprinting. Methods in Molecular Biology, vol 2140. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0520-2_14
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DOI: https://doi.org/10.1007/978-1-0716-0520-2_14
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