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Expressed Protein Ligation: General Experimental Protocols

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Book cover Expressed Protein Ligation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2133))

Abstract

Expressed protein ligation allows for the attachment of a chemically labeled peptide to the N- or C-terminus of a recombinant protein. In this book chapter, the practical considerations involved in using this protein engineering technology are described. In particular, approaches used to design optimal ligation sites are discussed. In addition, several methods used to generate the reactive fragments required for EPL are highlighted in practical details. Finally, strategies that one can implement to achieve efficient ligation reactions are presented.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA

    Joshua Diaz & Jean-Philippe Pellois

  2. Department of Chemistry, Texas A&M University, College Station, TX, USA

    Jean-Philippe Pellois

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  1. Joshua Diaz
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  2. Jean-Philippe Pellois
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Correspondence to Jean-Philippe Pellois .

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Editors and Affiliations

  1. ProteoDesign, Barcelona, Barcelona, Spain

    Miquel Vila-Perelló

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Diaz, J., Pellois, JP. (2020). Expressed Protein Ligation: General Experimental Protocols. In: Vila-Perelló, M. (eds) Expressed Protein Ligation. Methods in Molecular Biology, vol 2133. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0434-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0434-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0433-5

  • Online ISBN: 978-1-0716-0434-2

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