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Liquid Biopsy for Investigation of Cancer DNA in Esophageal Squamous Cell Carcinoma

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Esophageal Squamous Cell Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2129))

Abstract

Early detection of cancer and the monitoring of cancer recurrence in treated patients are significant challenges in esophageal squamous cell carcinoma (ESCC). Liquid biopsy is the identification of tumor biomarkers from minimally invasive samples of biological fluids, including urine, blood, stool, saliva, or cerebrospinal fluid. Liquid biopsy offers a potential solution to the problems of detection and surveillance as DNA shed from cancer cells as cell-free DNA or in exosomes can be detected in body fluids. By detecting these DNAs, we can identify the presence of cancer-associated mutations for basic detection, as well as to obtain information on the recurrence and evolution of disease following initial treatment. These sources of information have the potential to significantly improve the management of patients with ESCC. In this chapter, we detail a method for the isolation of cell-free DNA from blood plasma and DNA associated with exosomes in blood from patients with esophageal squamous cell carcinomas.

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Author information

Authors and Affiliations

  1. Genomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia

    Robert A. Smith

  2. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia

    Alfred K. Lam

Authors
  1. Robert A. Smith
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  2. Alfred K. Lam
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Corresponding author

Correspondence to Robert A. Smith .

Editor information

Editors and Affiliations

  1. Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, QLD, Australia

    Alfred K. Lam

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Smith, R.A., Lam, A.K. (2020). Liquid Biopsy for Investigation of Cancer DNA in Esophageal Squamous Cell Carcinoma. In: Lam, A. (eds) Esophageal Squamous Cell Carcinoma. Methods in Molecular Biology, vol 2129. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0377-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0377-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0376-5

  • Online ISBN: 978-1-0716-0377-2

  • eBook Packages: Springer Protocols

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