Skip to main content

Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

  • Protocol
  • First Online:
  • 725 Accesses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2126))

Abstract

Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given intravenously or intraperitoneally, followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Kaaru E et al (2016) Molecular imaging in preclinical models of IBD with nuclear imaging techniques: state-of-the-art and perspectives. Inflamm Bowel Dis 22(10):2491–2498

    Article  Google Scholar 

  2. von der Haar K et al (2015) Lost signature: progress and failures in in vivo tracking of implanted stem cells. Appl Microbiol Biotechnol 99(23):9907–9922

    Article  Google Scholar 

  3. Hampton MB, Kettle AJ, Winterbourn CC (1998) Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. Blood 92(9):3007–3017

    Article  CAS  Google Scholar 

  4. Treffers LW et al (2016) Neutrophils in cancer. Immunol Rev 273(1):312–328

    Article  CAS  Google Scholar 

  5. Klebanoff SJ (2005) Myeloperoxidase: friend and foe. J Leukoc Biol 77(5):598–625

    Article  CAS  Google Scholar 

  6. Chen WT, Tung CH, Weissleder R (2004) Imaging reactive oxygen species in arthritis. Mol Imaging 3(3):159–162

    Article  CAS  Google Scholar 

  7. Lundqvist H, Dahlgren C (1996) Isoluminol-enhanced chemiluminescence: a sensitive method to study the release of superoxide anion from human neutrophils. Free Radic Biol Med 20(6):785–792

    Article  CAS  Google Scholar 

  8. Gross S et al (2009) Bioluminescence imaging of myeloperoxidase activity in vivo. Nat Med 15(4):455–461

    Article  CAS  Google Scholar 

  9. Alshetaiwi HS et al (2013) Luminol-based bioluminescence imaging of mouse mammary tumors. J Photochem Photobiol B 127:223–228

    Article  CAS  Google Scholar 

  10. Davis RW et al (2019) Luminol Chemiluminescence reports photodynamic therapy-generated neutrophil activity in vivo and serves as a biomarker of therapeutic efficacy. Photochem Photobiol 95(1):430–438

    Article  CAS  Google Scholar 

  11. Breyne K et al (2017) Preconditioning with lipopolysaccharide or Lipoteichoic acid protects against Staphylococcus aureus mammary infection in mice. Front Immunol 8:833

    Article  Google Scholar 

  12. Veziant J et al (2016) Association of colorectal cancer with pathogenic Escherichia coli: focus on mechanisms using optical imaging. World J Clin Oncol 7(3):293–301

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tej B. Shrestha .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Shrestha, T.B., Basel, M.T. (2020). Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations. In: Basel, M., Bossmann, S. (eds) Cell Tracking. Methods in Molecular Biology, vol 2126. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0364-2_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0364-2_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0363-5

  • Online ISBN: 978-1-0716-0364-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics