Abstract
Brain tumors can prove difficult to diagnose and successfully treat. Gliomas, and in particular glioblastomas, are the most common type of primary brain tumor. The most difficult part about treating these tumors is the fact that they are able to migrate through the extracellular space inside the brain. Recurrence is also highly possible due to their invasive nature, leading to the destruction of nearby tissues. The migratory nature of these tumors makes imaging difficult. To combat this, antibodies can be conjugated to the surface of nanoparticles such as superparamagnetic iron oxide (SPIO) nanoparticles to help target the immune cells. This creates a unique bimodal system that is able to detect the brain cancer cells and assist tumor surgery in conjunction with magnetic resonance imaging (MRI).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mercurio L, Ajmone-Cat MA, Cecchetti S et al (2016) Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model. J Exp Clin Cancer Res 35:1–15
Richard S, Boucher M, Herbet A et al (2015) Endothelin B receptors targeted by iron oxide nanoparticles functionalized with a specific antibody: toward immunoimaging of brain tumors. J Mater Chem B 3:2939–2942
Cruz LJ, Tacken PJ, Zeelenberg IS et al (2014) Tracking targeted bimodal Nanovaccines: immune responses and routing in cells, tissue, and whole organism. Mol Pharm 11:4299–4313
Chen Y-C, Wen S, Shang S-A et al (2014) Magnetic resonance and near-infrared imaging using a novel dual-modality nano-probe for dendritic cell tracking in vivo. Cytotherapy 16:699–710
Zhou X, Luo B, Kang K et al (2019) Multifunctional luminescent immuno-magnetic nanoparticles: toward fast, efficient, cell-friendly capture and recovery of circulating tumor cells. J Mater Chem B 7:393–400
Pirko I, Johnson A, Ciric B et al (2004) In vivo magnetic resonance imaging of immune cells in the central nervous system with superparamagnetic antibodies. FASEB J 18:179–182
Gao J, Gu H, Xu B (2010) ChemInform abstract: multifunctional magnetic nanoparticles: design, synthesis, and biomedical applications. ChemInform 42(8):1097–1107
Hu R, Wang Y, Liu X et al (2013) Rational design of multimodal and multifunctional InP quantum dot nanoprobes for cancer: in vitro and in vivo applications. RSC Adv 3:8495
Weiner LM, Surana R, Wang S (2010) Monoclonal antibodies: versatile platforms for cancer immunotherapy. Nat Rev Immunol 10:317–327
Thierry B, Al-Ejeh F, Brown MP et al (2008) Immunotargeting of functional nanoparticles for MRI detection of apoptotic tumor cells. Adv Mater 21:541–545
Integra Biosciences Corporation. CELLine Disposable Bioreactor for Efficient Protein Expression. http://www.genetixbiotech.com/literature/CELLINE.pdf
Proteus Corporation. Protein Antibody Purification Handbook. https://www.bio-rad-antibodies.com/static/2015/proteus/protein-a-handbook.pdf
PerkinElmer Corporation. Better Characterizations Start with Better Controls. https://www.perkinelmer.com/labsolutions/resources/docs/BRO_TGA_8000_Brochure_012114A_02.pdf
Etoh N, Kondoh M, Ohnishi J et al (1994) Characterization of recombinant Endothelin receptor type B overexpressed in Chinese hamster ovary cells. Biomed Res 15:299–309
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Eschliman, K., Bossmann, S.H. (2020). Antibody-Targeted Magnetic Nanoparticles to Track Immune Cells In Vivo. In: Basel, M., Bossmann, S. (eds) Cell Tracking. Methods in Molecular Biology, vol 2126. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0364-2_12
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0364-2_12
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0363-5
Online ISBN: 978-1-0716-0364-2
eBook Packages: Springer Protocols