Abstract
Biolistic delivery of biomolecular cargoes to plants with micron-scale projectiles is a well-established technique in plant biotechnology. However, the relatively large micron-scale biolistic projectiles can result in tissue damage, low regeneration efficiency, and create difficulties for the biolistic transformation of isomorphic small cells or subcellular target organelles (i.e., mitochondria and plastids). As an alternative to micron-sized carriers, nanomaterials provide a promisingĀ approach for biomolecule delivery to plants. While most studies exploring nanoscale biolistic carriers have been carried out in animal cells and tissues, which lack a cell wall, we can nonetheless extrapolate their utility for nanobiolistic delivery of biomolecules in plant targets. Specifically, nanobiolistics has shown promising results for use in animal systems, in which nanoscale projectiles yield lower levels of cell and tissue damage while maintaining similar transformation efficiencies as their micron-scale counterparts. In this chapter, we specifically discuss biolistic delivery of nanoparticles for plant genetic transformation purposes and identify the figures of merit requiring optimization for broad-scale implementation of nanobiolistics in plant genetic transformations.
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References
Subcommittee N (2007) The national nanotechnology initiative. Nanotechnology. https://doi.org/10.4135/9781412972093.n338
Johlin E, Al-Obeidi A, Nogay G, Stuckelberger M, Buonassisi T, Grossman JC (2016) Nanohole structuring for improved performance of hydrogenated Amorphous silicon photovoltaics. ACS Appl Mater Interfaces 8:15169ā15176
Lee YM, Lee D, Kim J, Park H, Kim WJ (2015) RPM peptide conjugated bioreducible polyethylenimine targeting invasive colon cancer. J Control Release 205:172ā180
LaVan DA, McGuire T, Langer R (2003) Small-scale systems for in vivo drug delivery. Nat Biotechnol 21:1184ā1191
Cavalcanti A, Shirinzadeh B, Freitas RA Jr, Hogg T (2007) Nanorobot architecture for medical target identification. Nanotechnology 19:15103
Zadegan RM, Norton ML (2012) Structural DNA nanotechnology: from design to applications. Int J Mol Sci 13:7149ā7162
Ghormade V, Deshpande MV, Paknikar KM (2011) Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotechnol Adv 29:792ā803
Thangavelu RM, Gunasekaran D, Jesse MI, Riyaz MSU, Sundarajan D, Krishnan K (2018) Nanobiotechnology approach using plant rooting hormone synthesized silver nanoparticle as ānanobulletsā for the dynamic applications in horticulture ā an in vitro and ex vitro study. Arab J Chem 11:48ā61
Barrena R, Casals E, ColĆ³n J, Font X, SĆ”nchez A, Puntes V (2009) Evaluation of the ecotoxicity of model nanoparticles. Chemosphere 75:850ā857
Arora S, Sharma P, Kumar S, Nayan R, Khanna PK, Zaidi MGH (2012) Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul 66:303ā310
Salama HMH (2012) Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). Int Res J Biotechnol 3:190ā197
Tian H, Chen J, Chen X (2013) Nanoparticles for gene delivery. Small 9:2034ā2044
Demirer GS, Okur AC, Kizilel S (2015) Synthesis and design of biologically inspired biocompatible iron oxide nanoparticles for biomedical applications. J Mater Chem B 3:7831ā7849
Laurent S, Saei AA, Behzadi S, Panahifar A, Mahmoudi M (2014) Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: opportunities and challenges. Expert Opin Drug Deliv 11:1449ā1470
Nazli C, Demirer GS, Yar Y, Acar HY, Kizilel S (2014) Targeted delivery of doxorubicin into tumor cells via MMP-sensitive PEG hydrogel-coated magnetic iron oxide nanoparticles (MIONPs). Colloids Surfaces B Biointerfaces 122:674ā683
Mu Q, Jeon M, Hsiao M-H, Patton VK, Wang K, Press OW, Zhang M (2015) Stable and efficient paclitaxel nanoparticles for targeted glioblastoma therapy. Adv Healthc Mater 4:1236ā1245
Jin S, Leach JC, Ye K (2009) Nanoparticle-mediated gene delivery. In: Foote RS, Lee JW (eds) Micro and nano technologies in bioanalysis: methods and protocols. Humana, Totowa, NJ, pp 547ā557
Pissuwan D, Niidome T, Cortie MB (2011) The forthcoming applications of gold nanoparticles in drug and gene delivery systems. J Control Release 149:65ā71
Gu Y-J, Cheng J, Lin C-C, Lam YW, Cheng SH, Wong W-T (2009) Nuclear penetration of surface functionalized gold nanoparticles. Toxicol Appl Pharmacol 237:196ā204
Gibson JD, Khanal BP, Zubarev ER (2007) Paclitaxel-functionalized gold nanoparticles. J Am Chem Soc 129:11653ā11661
Shiotani A, Mori T, Niidome T, Niidome Y, Katayama Y (2007) Stable incorporation of gold nanorods into N-isopropylacrylamide hydrogels and their rapid shrinkage induced by near-infrared laser irradiation. Langmuir 23:4012ā4018
Han G, Martin CT, Rotello VM (2006) Stability of gold nanoparticle-bound DNA toward biological, physical, and chemical agents. Chem Biol Drug Des 67:78ā82
Han G, Chari NS, Verma A, Hong R, Martin CT, Rotello VM (2005) Controlled recovery of the transcription of nanoparticle-bound DNA by intracellular concentrations of glutathione. Bioconjug Chem 16:1356ā1359
Pezzoli D, Kajaste-Rudnitski A, Chiesa R, Candiani G (2013) Lipid-based nanoparticles as nonviral gene delivery vectors. In: Bergese P, Hamad-Schifferli K (eds) Nanomaterial interfaces in biology: methods and protocols. Humana, Totowa, NJ, pp 269ā279
Blanco E, Shen H, Ferrari M (2015) Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat Biotechnol 33:941ā951
Hui SW, Langner M, Zhao Y-L, Ross P, Hurley E, Chan K (1996) The role of helper lipids in cationic liposome-mediated gene transfer. Biophys J 71:590ā599
Chatin B, MĆ©vel M, DevalliĆØre J, Dallet L, Haudebourg T, Peuziat P, Colombani T, Berchel M, Lambert O, Edelman A, Pitard B (2015) Liposome-based formulation for intracellular delivery of functional proteins. Mol Ther Nucleic Acids 4:e244
Wang M, Zuris JA, Meng F, Rees H, Sun S, Deng P, Han Y, Gao X, Pouli D, Wu Q, Georgakoudi I, Liu DR, Xu Q (2016) Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles. Proc Natl Acad Sci U S A 113:2868ā2873
Torchilin VP (2014) Multifunctional, stimuli-sensitive nanoparticulate systems for drug delivery. Nat Rev Drug Discov 13:813ā827
Nitta KS, Numata K (2013) Biopolymer-based nanoparticles for drug/gene delivery and tissue engineering. Int J Mol Sci 14:1629ā1654
Li J, Liang H, Liu J, Wang Z (2018) Poly (amidoamine) (PAMAM) dendrimer mediated delivery of drug and pDNA/siRNA for cancer therapy. Int J Pharm 546:215ā225
Pasupathy K, Lin S, Hu Q, Luo H, Ke PC (2008) Direct plant gene delivery with a poly(amidoamine) dendrimer. Biotechnol J 3:1078ā1082
Hartono SB, Phuoc NT, Yu M, Jia Z, Monteiro MJ, Qiao S, Yu C (2014) Functionalized large pore mesoporous silica nanoparticles for gene delivery featuring controlled release and co-delivery. J Mater Chem B 2:718ā726
Torney F, Trewyn BG, Lin VSY, Wang K (2007) Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotechnol 2:295ā300
Karimi M, Solati N, Ghasemi A, Estiar MA, Hashemkhani M, Kiani P, Mohamed E, Saeidi A, Taheri M, Avci P, Aref AR, Amiri M, Baniasadi F, Hamblin MR (2015) Carbon nanotubes part II: a remarkable carrier for drug and gene delivery. Expert Opin Drug Deliv 12:1089ā1105
Demirer GS, Landry MP (2017) Delivering genes to plants. Chem Eng Prog 113:40ā45
Bhirde AA, Patel V, Gavard J, Zhang G, Sousa AA, Masedunskas A, Leapman RD, Weigert R, Gutkind JS, Rusling JF (2009) Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drug delivery. ACS Nano 3:307ā316
Heister E, Neves V, TĆ®lmaciu C, Lipert K, BeltrĆ”n VS, Coley HM, Silva SRP, McFadden J (2009) Triple functionalisation of single-walled carbon nanotubes with doxorubicin, a monoclonal antibody, and a fluorescent marker for targeted cancer therapy. Carbon 47:2152ā2160
Liu Z, Chen K, Davis C, Sherlock S, Cao Q, Chen X, Dai H (2008) Drug delivery with carbon nanotubes for in vivo cancer treatment. Cancer Res 68:6652ā6660
Shi Kam NW, Jessop TC, Wender PA, Dai H (2004) Nanotube molecular transporters: internalization of carbon nanotubeā protein conjugates into mammalian cells. J Am Chem Soc 126:6850ā6851
Kam NWS, Liu Z, Dai H (2006) Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. Angew Chem 118:591ā595
Dong H, Ding L, Yan F, Ji H, Ju H (2011) The use of polyethylenimine-grafted graphene nanoribbon for cellular delivery of locked nucleic acid modified molecular beacon for recognition of microRNA. Biomaterials 32:3875ā3882
Qin W, Yang K, Tang H, Tan L, Xie Q, Ma M, Zhang Y, Yao S (2011) Improved GFP gene transfection mediated by polyamidoamine dendrimer-functionalized multi-walled carbon nanotubes with high biocompatibility. Colloids Surfaces B Biointerfaces 84:206ā213
Karmakar A, Bratton SM, Dervishi E, Ghosh A, Mahmood M, Xu Y, Saeed LM, Mustafa T, Casciano D, Radominska-Pandya A, Biris AS (2011) Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells. Int J Nanomedicine 6:1045ā1055
Wu Y, Phillips JA, Liu H, Yang R, Tan W (2008) Carbon nanotubes protect DNA strands during cellular delivery. ACS Nano 2:2023ā2028
Zheng M, Jagota A, Semke ED, Diner BA, McLean RS, Lustig SR, Richardson RE, Tassi NG (2003) DNA-assisted dispersion and separation of carbon nanotubes. Nat Mater 2:338ā342
Wang H, Koleilat GI, Liu P, JimĆ©nez-OsĆ©s G, Lai YC, Vosgueritchian M, Fang Y, Park S, Houk KN, Bao Z (2014) High-yield sorting of small-diameter carbon nanotubes for solar cells and transistors. ACS Nano 8:2609ā2617
Wong MH, Misra RP, Giraldo JP, Kwak SY, Son Y, Landry MP, Swan JW, Blankschtein D, Strano MS (2016) Lipid exchange envelope penetration (LEEP) of nanoparticles for plant engineering: a universal localization mechanism. Nano Lett 16:1161ā1172
Giraldo JP, Landry MP, Faltermeier SM, McNicholas TP, Iverson NM, Boghossian AA, Reuel NF, Hilmer AJ, Sen F, Brew JA, Strano MS (2014) Plant nanobionics approach to augment photosynthesis and biochemical sensing. Nat Mater 13:400ā408
Demirer GS, Zhang H, Matos J, Goh NS, Cunningham FJ, Sung Y, Chang R, Aditham AJ, Chio L, Cho MJ, Staskawicz B, Landry MP (2018) High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants. Nat Nanotechnol 14:456ā464
Cunningham FJ, Goh NS, Demirer GS, Matos JL, Landry MP (2018) Nanoparticle-mediated delivery towards advancing plant genetic engineering. Trends Biotechnol 36:882ā897
Wang P, Lombi E, Zhao F-JJ, Kopittke PM (2016) Nanotechnology: a new opportunity in plant sciences. Trends Plant Sci 21:699ā712
Sidorov VA, Kasten D, Pang S, Hajdukiewicz PT, Staub JM, Nehra NS (1999) Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19:209ā216
Kwak S-Y, Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua NH, Strano MS (2016) Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447ā455
Kreyling WG, Semmler-Behnke M, Chaudhry Q (2010) A complementary definition of nanomaterial. Nano Today 5:165ā168
Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Kumar DS (2010) Nanoparticulate material delivery to plants. Plant Sci 179:154ā163
Parisi C, Vigani M, RodrĆguez-Cerezo E (2015) Agricultural nanotechnologies: what are the current possibilities? Nano Today 10:124ā127
Miralles P, Church TL, Harris AT (2012) Toxicity, uptake, and translocation of engineered nanomaterials in vascular plants. Environ Sci Technol 46:9224ā9239
PĆ©rez-de-Luque A (2017) Interaction of nanomaterials with plants: what do we need for real applications in agriculture? Front Environ Sci 5:12
Zhang F, Wang R, Xiao Q, Wang Y, Zhang J (2006) Effects of slow/controlled-release fertilizer cemented and coated by nano-materials on biology. II Effects of slow/controlled-release fertilizer cemented and coated by nano-materials on plants. Nanoscience 11:18ā26
CaƱas JE, Long M, Nations S, Vadan R, Dai L, Luo M, Ambikapathi R, Lee EH, Olszyk D (2008) Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species. Environ Toxicol Chem 27:1922ā1931
Stampoulis D, Sinha SK, White JC (2009) Assay-dependent phytotoxicity of nanoparticles to plants. Environ Sci Technol 43:9473ā9479
Zhu H, Han J, Xiao JQ, Jin Y (2008) Uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. J Environ Monit 10:713ā717
GrĆ¼n M, Lauer I, Unger KK (1997) The synthesis of micrometer- and submicrometer-size spheres of ordered mesoporous oxide MCM-41. Adv Mater 9:254ā257
Wu S-H, Mou C-Y, Lin H-P (2013) Synthesis of mesoporous silica nanoparticles. Chem Soc Rev 42:3862ā3875
Slowing I, Trewyn BG, Lin VS (2006) Effect of surface functionalization of MCM-41-type mesoporous silica nanoparticles on the endocytosis by human cancer cells. J Am Chem Soc 128:14792ā14793
Lim MH, Blanford CF, Stein A (1998) Synthesis of ordered microporous silicates with organosulfur surface groups and their applications as solid acid catalysts. Chem Mater 102:467ā470
Lai CY, Trewyn BG, Jeftinija DM, Jeftinija K, Xu S, Jeftinija S, Lin VS (2003) A mesoporous silica nanosphere-based carrier system with chemically removable CdS nanoparticle caps for stimuli-responsive controlled release of neurotransmitters and drug molecules. J Am Chem Soc 125:4451ā4459
Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci 241:20ā22
Brust M, Walker M, Bethell D, Schiffrin DJ, Whyman R (1994) Synthesis of thiol-derivatised gold nanoparticles in a two-phase liquidāliquid system. J Chem Soc Chem Commun 7:801ā802
Zhao P, Li N, Astruc D (2013) State of the art in gold nanoparticle synthesis. Coord Chem Rev 257:638ā665
PĆ©rez-Juste J, Pastoriza-Santos I, Liz-MarzĆ”n LM, Mulvaney P (2005) Gold nanorods: synthesis, characterization and applications. Coord Chem Rev 249:1870ā1901
Bhattacharjee S (2016) DLS and zeta potential ā what they are and what they are not? J Control Release 235:337ā351
Klein TM, Wolf ED, Wu R, Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327:70ā73
Roizenblatt R, Weiland JD, Carcieri S, Qiu G, Behrend M, Humayun MS, Chow RH (2006) Nanobiolistic delivery of indicators to the living mouse retina. J Neurosci Methods 153:154ā161
Arsenault J, OāBrien JA (2013) Optimized heterologous transfection of viable adult organotypic brain slices using an enhanced gene gun. BMC Res Notes 6:544
OāBrien JA, Lummis SC (2011) Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles. BMC Biotechnol 11:66
Lee P-W, Peng S-F, Su C-J, Mi FL, Chen HL, Wei MC, Lin HJ, Sung HW (2008) The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery. Biomaterials 29:742ā751
Lee P-W, Hsu S-H, Tsai J-S, Chen FR, Huang PJ, Ke CJ, Liao ZX, Hsiao CW, Lin HJ, Sung HW (2010) Multifunctional core-shell polymeric nanoparticles for transdermal DNA delivery and epidermal Langerhans cells tracking. Biomaterials 31:2425ā2434
Huang HN, Li TL, Chan YL, Chen CL, Wu CJ (2009) Transdermal immunization with low-pressure-gene-gun mediated chitosan-based DNA vaccines against Japanese encephalitis virus. Biomaterials 30:6017ā6025
Raji JA, Frame B, Little D, Santoso TJ, Wang K (2018) Agrobacterium- and biolistic-mediated transformation of maize b104 inbred. In: Lagrimini LM (ed) Maize: methods and protocols. Springer, New York, NY, pp 15ā40
Svitashev S, Schwartz C, Lenderts B, Young JK, Mark Cigan A (2016) Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes. Nat Commun 7:13274
Ismagul A, Yang N, Maltseva E, Iskakova G, Mazonka I, Skiba Y, Bi H, Eliby S, Jatayev S, Shavrukov Y, Borisjuk N, Langridge P (2018) A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions. BMC Plant Biol 18:135
Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, Qiu JL (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol 32:947ā951
Kumari M, Rai AK, Devanna BN, Singh PK, Kapoor R, Rajashekara H, Prakash G, Sharma V, Sharma TR (2017) Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae. Plant Cell Rep 36:1747ā1755
Srivastava V, Underwood JL, Zhao S (2017) Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome. Plant Cell Tissue Organ Cult 129:153ā160
Chaithra N, Gowda RPH, Guleria N (2015) Transformation of tomato with Cry2ax1 by biolistic gun method for fruit borer resistance. Int J Agric Environ Biotechnol 8:795ā803
Kumar N, Galli M, Ordon J, Stuttmann J, Kogel KH, Imani J (2018) Further analysis of barley MORC1 using a highly efficient RNA-guided Cas9 gene-editing system. Plant Biotechnol J 16:1892ā1903
Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA (2015) Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnol 15:16
Rafsanjani MS, Alvari A, Samim M, Hejazi MA, Abdin MZ (2012) Application of novel nanotechnology strategies in plant biotransformation: a contemporary overview. Recent Pat Biotechnol 6:69ā79
Zalewski W, Orczyk W, Gasparis S, Nadolska-Orczyk A (2012) HvCKX2 gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes. BMC Plant Biol 12:206
Alok A, Sharma S, Kumar J, Verma S, Sood H (2017) Engineering in plant genome using Agrobacterium: progress and future. In: Kalia VC, Saini AK (eds) Metabolic engineering for bioactive compounds: Strategies and processes. Springer, Singapore, pp 91ā111
Anand A, Trick HN, Gill BS, Muthukrishnan S (2003) Stable transgene expression and random gene silencing in wheat. Plant Biotechnol J 1:241ā251
Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci U S A 95:7203ā7208
Tassy C, Partier A, Beckert M, Feuillet C, Barret P (2014) Biolistic transformation of wheat: increased production of plants with simple insertions and heritable transgene expression. Plant Cell Tissue Organ Cult 119:171ā181
Martin-Ortigosa S, Valenstein JS, Lin VS-Y, Trewyn BG, Wang K (2012) Gold functionalized mesoporous silica nanoparticle mediated protein and DNA codelivery to plant cells via the biolistic method. Adv Funct Mater 22:3576ā3582
Martin-Ortigosa S, Valenstein JS, Sun W, Moeller L, Fang N, Trewyn BG, Lin VS, Wang K (2012) Parameters affecting the efficient delivery of mesoporous silica nanoparticle materials and gold nanorods into plant tissues by the biolistic method. Small 8:413ā422
Martin-Ortigosa S, Peterson DJ, Valenstein JS, Lin VS, Trewyn BG, Lyznik LA, Wang K (2014) Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision. Plant Physiol 164:537ā547
Mortazavi SE, Zohrabi Z (2018) Biolistic co-transformation of rice using gold nanoparticles. Iran Agric Res 37:75ā82
Okuzaki A, Kida S, Watanabe J, Hirasawa I, Tabei Y (2013) Efficient plastid transformation in tobacco using small gold particles (0.07ā0.3 Ī¼m). Plant Biotechnol 30:65ā72
Demirer GS, Zhang H, Goh NS, Pinals RL, Chang R, Landry MP (2019). Carbon Nanocarriers Deliver siRNA to Intact Plant Cells for Efficient Gene Knockdown. bioRxiv, 564427
Zhang H, Demirer GS, Zhang H, Ye T, Goh NS, Aditham AJ, Cunningham FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad Sci 116(15):7543ā7548
Acknowledgments
The authors acknowledge support from a Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI), a Beckman Foundation Young Investigator Award, a USDA AFRI award, a grant from the Gordon and Betty Moore Foundation, a USDA NIFA award,Ā an NIH MIRA award, support from the Chan-Zuckerberg foundation, and an FFAR New Innovator Award (to M.P.L). F.J.C is supported by an NSF Graduate Research Fellowship, N.S.G is supported by a FFAR Fellowship,Ā and G.S.D. is supported by a Schlumberger Foundation Faculty for the Future Fellowship.
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Cunningham, F.J., Demirer, G.S., Goh, N.S., Zhang, H., Landry, M.P. (2020). Nanobiolistics: An Emerging Genetic Transformation Approach. In: Rustgi, S., Luo, H. (eds) Biolistic DNA Delivery in Plants. Methods in Molecular Biology, vol 2124. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0356-7_7
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