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Isolation of Swine Influenza A Virus in Cell Cultures and Embryonated Chicken Eggs

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Animal Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2123))

Abstract

Influenza virus isolation is a procedure to obtain a live and infectious virus that can be used for antigenic characterization, pathogenesis investigation, vaccine production, and so on. Embryonated chicken egg inoculation is traditionally considered the “gold standard” method for influenza virus isolation and propagation. However, many primary cells and continuous cell lines have also been examined or developed for influenza virus isolation and replication. Specifically, influenza A virus in swine (IAV-S) isolation and propagation has been attempted and compared in embryonated chicken eggs, some primary porcine cells, and a number of continuous cell lines. Currently, Madin-Darby canine kidney (MDCK) cells remain the most commonly used cell line for the isolation, propagation, and titration of IAV-S. Virus isolation in embryonated chicken eggs or in different cell lines offers alternative approaches when IAV-S isolation in MDCK cells is unsuccessful. Optimal specimens for IAV-S isolation includes nasal swabs, nasopharyngeal swabs, oral fluids, bronchoalveolar lavage, lung tissues, and so on. In this chapter, we describe the procedures of sample processing, IAV-S isolation in MDCK cells and in embryonated chicken eggs, as well as the methods used for confirming the virus isolation results.

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Authors and Affiliations

  1. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA

    Jianqiang Zhang & Phillip C. Gauger

Authors
  1. Jianqiang Zhang
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  2. Phillip C. Gauger
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Correspondence to Jianqiang Zhang .

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Editors and Affiliations

  1. Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA

    Erica Spackman

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© 2020 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

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Zhang, J., Gauger, P.C. (2020). Isolation of Swine Influenza A Virus in Cell Cultures and Embryonated Chicken Eggs. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 2123. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0346-8_20

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  • DOI: https://doi.org/10.1007/978-1-0716-0346-8_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0345-1

  • Online ISBN: 978-1-0716-0346-8

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