Abstract
Neuroendocrine hormones are recognized as important mediators of inflammation that participate in the regulation of the magnitude and length of the immune response. It was demonstrated that endogenous glucocorticoids control the function of innate lymphoid cells (ILCs), and this regulatory mechanism is both cell type- and tissue-specific and is required for host protection during infections. We describe here how to analyze in vitro the effects of corticosterone on murine ILCs, using flow cytometry. The protocols described allow for the identification of the specific combination of stimuli with which glucocorticoids cooperate to regulate the function of ILCs. These methods are instrumental to understanding the molecular mechanisms downstream of glucocorticoid receptor activation and can explain the tissue specificity of ILC response to glucocorticoids.
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References
Weikum ER, Knuesel MT, Ortlund EA et al (2017) Glucocorticoid receptor control of transcription: precision and plasticity via allostery. Nat Rev Mol Cell Biol 18:159–174
Cain DW, Cidlowski JA (2017) Immune regulation by glucocorticoids. Nat Rev Immunol 17:233–247
Quatrini L, Wieduwild E, Guia S et al (2017) Host resistance to endotoxic shock requires the neuroendocrine regulation of group 1 innate lymphoid cells. J Exp Med 214:3531–3541
Quatrini L, Wieduwild E, Escaliere B et al (2018) Endogenous glucocorticoids control host resistance to viral infection through the tissue-specific regulation of PD-1 expression on NK cells. Nat Immunol 19:954–962
Vivier E, Tomasello E, Baratin M et al (2008) Functions of natural killer cells. Nat Immunol 9:503–510
Shapiro HM (1993) Trends and developments in flow cytometry instrumentation. Ann N Y Acad Sci 677:155–166
Assenmacher M, Schmitz J, Radbruch A (1994) Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells. Eur J Immunol 24:1097–1101
Elson LH, Nutman TB, Metcalfe DD et al (1995) Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27− lymphocyte subpopulation. J Immunol 154:4294–4301
Jung T, Schauer U, Heusser C et al (1993) Detection of intracellular cytokines by flow cytometry. J Immunol Methods 159:197–207
Prussin C, Metcalfe DD (1995) Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods 188:117–128
Sander B, Hoiden I, Andersson U et al (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods 166:201–214
Carlyle JR, Mesci A, Ljutic B et al (2006) Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol 176:7511–7524
Acknowledgments
S. Ugolini lab is supported by institutional grants from INSERM, CNRS, Aix-Marseille University and Marseille-Immunopole to the CIML and received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program, under grant agreement No. 648768; from the Agence Nationnale de la Recherche (ANR) (No. ANR-14-CE14-0009-01) and from the ARC foundation (No. PGA120140200817). L. Quatrini has received funding from AIRC and from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 800924.
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Quatrini, L., Ugolini, S. (2020). Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function. In: Amarnath, S. (eds) Innate Lymphoid Cells . Methods in Molecular Biology, vol 2121. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0338-3_8
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DOI: https://doi.org/10.1007/978-1-0716-0338-3_8
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Publisher Name: Humana, New York, NY
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