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Nanoparticles in Biology and Medicine pp 91–98Cite as

Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2118))

Abstract

Therapeutic gene delivery systems offer the potential for the treatment of a range of inherited and acquired inherited diseases. In contrast with viral gene vectors, the nonviral gene vectors provide a safer alternative and additional advantages such as the improved delivery efficiency, low cost, and often unlimited capacity to package DNA. Here we describe the preparation of a nonviral gene delivery technique based on lipid–peptide–DNA (LPD) complexes. The size of LPD particles is in the nanometer range. The use of these nanoparticulate LPDs results in high efficiency transfections and a high level of gene expression in vitro. LPDs provide a convenient and efficient tool for gene delivery in gene therapy.

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Acknowledgments

The manuscript was edited by Mikhail Soloviev.

Author information

Authors and Affiliations

  1. Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    Fan Zhang

  2. Biomanufacturing Research Centre, School of Mechanical and Electronic Engineering, Soochow University, Suzhou, China

    Hao-Ying Li

Authors
  1. Fan Zhang
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  2. Hao-Ying Li
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Corresponding author

Correspondence to Hao-Ying Li .

Editor information

Editors and Affiliations

  1. College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, UK

    Enrico Ferrari

  2. Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK

    Mikhail Soloviev

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Zhang, F., Li, HY. (2020). Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection. In: Ferrari, E., Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 2118. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0319-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0319-2_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0318-5

  • Online ISBN: 978-1-0716-0319-2

  • eBook Packages: Springer Protocols

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