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Molecular Toxicology Protocols pp 163–176Cite as

Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2102))

Abstract

Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent dyes, and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.

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Author information

Authors and Affiliations

  1. Department of Occupational and Environmental Health Sciences, School of Public Health, West Virginia University, Morgantown, WV, USA

    Weimin Gao

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  1. Weimin Gao
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Correspondence to Weimin Gao .

Editor information

Editors and Affiliations

  1. Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA

    Phouthone Keohavong

  2. Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Lubbock, TX, USA

    Kamaleshwar P. Singh

  3. Department of Occupational and Environmental Health Sciences, School of Public Health West Virginia University, Morgantown, WV, USA

    Weimin Gao

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Gao, W. (2020). Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis. In: Keohavong, P., Singh, K., Gao, W. (eds) Molecular Toxicology Protocols. Methods in Molecular Biology, vol 2102. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0223-2_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0223-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0222-5

  • Online ISBN: 978-1-0716-0223-2

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