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Detection of Intact Transcription Factors in Human Neutrophils

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Neutrophil

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2087))

Abstract

The crucial contribution of neutrophils to innate immunity extends well beyond their traditional role as professional phagocytes. Indeed, it is now well established that neutrophils generate a plethora of inflammatory cytokines and chemokines that are profoundly involved in the onset and evolution of the inflammatory reaction. Several recent studies have shown that neutrophils can represent an important source of inflammatory cytokines in pathophysiological settings. The inflammatory and immunomodulatory cytokines produced by neutrophils are generally encoded by immediate-early response genes, which in turn depend on the activation of transcription factors such as those belonging to the nuclear factor κB (NF-κB) and signal transducers and activators of transcription (STAT) families. We have shown in the past that the expression of such factors is induced in neutrophils stimulated by physiological agonists. However, the detection of intact (i.e., undegraded) transcription factors in neutrophils requires special precautions and a specially designed protocol, due to the huge amounts of endogenous proteases present in these cells. This protocol is the focus of this chapter.

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Acknowledgments

This work was supported by grants to PPMcD from the Canadian Institutes for Health Research and the Arthritis Foundation of Canada, and to RDY from the National Institutes of Health. PPMcD is a scholar of Fonds de la recherche en santé du Québec (FRSQ).

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Authors and Affiliations

  1. Pulmonary Division, Medicine Faculty, Université de Sherbrooke, Sherbrooke, QC, Canada

    Patrick P. McDonald

  2. Kobilka Institute of Innovative Drug Discovery, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, China

    Richard D. Ye

Authors
  1. Patrick P. McDonald
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  2. Richard D. Ye
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Corresponding author

Correspondence to Richard D. Ye .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA

    Mark T. Quinn

  2. Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Frank R. DeLeo

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McDonald, P.P., Ye, R.D. (2020). Detection of Intact Transcription Factors in Human Neutrophils. In: Quinn, M., DeLeo, F. (eds) Neutrophil. Methods in Molecular Biology, vol 2087. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0154-9_20

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  • DOI: https://doi.org/10.1007/978-1-0716-0154-9_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0153-2

  • Online ISBN: 978-1-0716-0154-9

  • eBook Packages: Springer Protocols

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