Abstract
Manufacturing chimeric antigen receptor (CAR)-modified T cells requires incorporation of the CAR transgene, for which viral vectors are most often used. Here, we describe the generation of CAR T cells using primary human T cells and a non–self-inactivating gammaretroviral vector encoding a CAR transgene. The gammaretroviral vector is produced by 293T cells transiently transfected with DNA plasmids encoding necessary components of the viral vector. The resulting viral particles efficiently infect activated T cells and integrate the CAR transgene into the genome of dividing cells for stable expression.
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Acknowledgments
The authors thank Catherine Gillespie for editing the chapter. This work was supported by the CPRIT Awards RP180810 and RP150611, and by the Leukemia and Lymphoma Society TRP Award.
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Mo, F., Mamonkin, M. (2020). Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors. In: Swiech, K., Malmegrim, K., Picanço-Castro, V. (eds) Chimeric Antigen Receptor T Cells. Methods in Molecular Biology, vol 2086. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0146-4_8
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DOI: https://doi.org/10.1007/978-1-0716-0146-4_8
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0146-4
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