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Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays

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Chimeric Antigen Receptor T Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2086))

Abstract

Immunotherapy using T cells modified with chimeric antigen receptor (CAR) has been proven effective in the treatment of leukemia and lymphomas resistant to chemotherapy. Recent clinical studies have shown excellent responses of CAR-T cells in a variety of B cell tumors. However, it is important to validate in vitro activity of these cells, though different sorts of assays, which are capable of measuring the cytotoxic potential of these cells. In this chapter, it will be pointed two methods to evaluate CAR-T cell killing potential against B cell malignancy cell lines.

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Acknowledgments

The authors acknowledge São Paulo Research Foundation—FAPESP (2015/19017-6, 2016/08374-5), the National Council for Scientific and Technological Development—CNPq (142406/2016-3), Research, Innovation, and Dissemination Centers—RIDC (2013/08135-2), the Coordination of Improvement of Higher Level Personnel – Capes (88887.140966/2017-00) and the National Institute of Science and Technology in Stem Cell and Cell Therapy—INCTC (465539/2014-9) for financial support. The authors also acknowledge financial support from Secretaria Executiva do Ministério da Saúde (SE/MS), Departamento de Economia da Saúde, Investimentos e Desenvolvimento (DESID/SE), Programa Nacional de Apoio à Atenção Oncológica (PRONON) Process 25000.189625/2016-16.

Author information

Authors and Affiliations

  1. Center for Cell-Based Therapy CTC, Regional Blood Center of Ribeirão Preto, University of São Paulo, São Paulo, Brazil

    Renata Nacasaki Silvestre, Pablo Diego Moço & Virgínia Picanço-Castro

Authors
  1. Renata Nacasaki Silvestre
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  2. Pablo Diego Moço
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  3. Virgínia Picanço-Castro
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Corresponding author

Correspondence to Renata Nacasaki Silvestre .

Editor information

Editors and Affiliations

  1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Kamilla Swiech

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Kelen Cristina Ribeiro Malmegrim

  3. Center for Cell-Based Therapy CTC Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Virgínia Picanço-Castro

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Nacasaki Silvestre, R., Moço, P.D., Picanço-Castro, V. (2020). Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays. In: Swiech, K., Malmegrim, K., Picanço-Castro, V. (eds) Chimeric Antigen Receptor T Cells. Methods in Molecular Biology, vol 2086. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0146-4_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0146-4_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0145-7

  • Online ISBN: 978-1-0716-0146-4

  • eBook Packages: Springer Protocols

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