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Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles

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Stem Cell Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2125))

Abstract

Therapy based on stem cells utilizes these cells in neurodegeneration, brain/spinal cord injury, and much recently in repairing of severe heart diseases. Owning to their stemness, these cells are the potential source of progenitors that can offer a therapeutic remedy to a variety of diseases and/or disorders. The ability of these cells to regenerate and differentiate into specified phenotypes has great utility in tissue regeneration applications. This chapter provides a detailed account for isolation of neural stem cells from the mice embryo. Furthermore, the fabrication of chitosan–tripolyphosphate/hyaluronic acid-based nanoparticles and evaluating their efficiency in inducing transfection in the isolated neural stem cells as an approach for the treatment of neurodegenerative disorders.

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References

  1. Mahla RS (2016) Stem cells applications in regenerative medicine and disease therapeutics. Int J Cell Biol 2016:6940283

    Article  Google Scholar 

  2. Trounson A, DeWitt ND (2016) Pluripotent stem cells progressing to the clinic. Nat Rev Mol Cell Biol 17(3):194

    Article  CAS  Google Scholar 

  3. Tao H, Han Z, Han ZC, Li Z (2016) Proangiogenic features of mesenchymal stem cells and their therapeutic applications. Stem Cells Int 2016:1314709

    Article  Google Scholar 

  4. Hyslop LA, Blakeley P, Craven L, Richardson J, Fogarty NM, Fragouli E, Lamb M, Wamaitha SE, Prathalingam N, Zhang Q (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534(7607):383

    Article  CAS  Google Scholar 

  5. Wankhade UD, Shen M, Kolhe R, Fulzele S (2016) Advances in adipose-derived stem cells isolation, characterization, and application in regenerative tissue engineering. Stem Cells Int 2016:3206807

    Article  Google Scholar 

  6. Rana D, Zreiqat H, Benkirane-Jessel N, Ramakrishna S, Ramalingam M (2017) Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med 11(4):942–965

    Article  CAS  Google Scholar 

  7. Dai R, Wang Z, Samanipour R, Koo K-I, Kim K (2016) Adipose-derived stem cells for tissue engineering and regenerative medicine applications. Stem Cells Int 2016:6737345

    PubMed  PubMed Central  Google Scholar 

  8. Mohammadi M, Jaafari M, Mirzaei H, Mirzaei H (2016) Mesenchymal stem cell: a new horizon in cancer gene therapy. Cancer Gene Ther 23(9):285

    Article  CAS  Google Scholar 

  9. Kim SU, De Vellis J (2009) Stem cell-based cell therapy in neurological diseases: a review. J Neurosci Res 87(10):2183–2200

    Article  CAS  Google Scholar 

  10. Myers TJ, Granero-Molto F, Longobardi L, Li T, Yan Y, Spagnoli A (2010) Mesenchymal stem cells at the intersection of cell and gene therapy. Expert Opin Biol Ther 10(12):1663–1679

    Article  CAS  Google Scholar 

  11. Lindvall O, Barker RA, Brüstle O, Isacson O, Svendsen CN (2012) Clinical translation of stem cells in neurodegenerative disorders. Cell Stem Cell 10(2):151–155

    Article  CAS  Google Scholar 

  12. Doss MX, Koehler CI, Gissel C, Hescheler J, Sachinidis A (2004) Embryonic stem cells: a promising tool for cell replacement therapy. J Cell Mol Med 8(4):465–473

    Article  Google Scholar 

  13. Watt FM, Huck WT (2013) Role of the extracellular matrix in regulating stem cell fate. Nat Rev Mol Cell Biol 14(8):467

    Article  CAS  Google Scholar 

  14. L Santos J, Pandita D, Rodrigues J, P Pego A, L Granja P, Tomás H (2011) Non-viral gene delivery to mesenchymal stem cells: methods, strategies and application in bone tissue engineering and regeneration. Curr Gene Ther 11(1):46–57

    Article  Google Scholar 

  15. McMahon J, Conroy S, Lyons M, Greiser U, O'shea C, Strappe P, Howard L, Murphy M, Barry F, O'brien T (2006) Gene transfer into rat mesenchymal stem cells: a comparative study of viral and nonviral vectors. Stem Cells Dev 15(1):87–96

    Article  CAS  Google Scholar 

  16. Zhu K, Wu M, Lai H, Guo C, Li J, Wang Y, Chen Y, Wang C, Shi J (2016) Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair. Biomaterials 74:188–199

    Article  CAS  Google Scholar 

  17. Park JS, Yang HN, Yi SW, Kim J-H, Park K-H (2016) Neoangiogenesis of human mesenchymal stem cells transfected with peptide-loaded and gene-coated PLGA nanoparticles. Biomaterials 76:226–237

    Article  CAS  Google Scholar 

  18. Tenkumo T, Vanegas Sáenz JR, Takada Y, Takahashi M, Rotan O, Sokolova V, Epple M, Sasaki K (2016) Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite–collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles. Genes Cells 21(7):682–695

    Article  CAS  Google Scholar 

  19. Das J, Choi Y-J, Yasuda H, Han JW, Park C, Song H, Bae H, Kim J-H (2016) Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation. Sci Rep 6:33784

    Article  Google Scholar 

  20. Xu S, De Becker A, Van Camp B, Vanderkerken K, Van Riet I (2010) An improved harvest and in vitro expansion protocol for murine bone marrow-derived mesenchymal stem cells. Biomed Res Int 2010:105940

    Google Scholar 

  21. Barry FP, Murphy JM (2004) Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 36(4):568–584

    Article  CAS  Google Scholar 

  22. Lindvall O, Kokaia Z (2006) Stem cells for the treatment of neurological disorders. Nature 441(7097):1094

    Article  CAS  Google Scholar 

  23. Lindvall O, Kokaia Z, Martinez-Serrano A (2004) Stem cell therapy for human neurodegenerative disorders—how to make it work. Nat Med 10(7s):S42

    Article  Google Scholar 

  24. Lindvall O, Kokaia Z (2010) Stem cells in human neurodegenerative disorders—time for clinical translation? J Clin Invest 120(1):29–40

    Article  CAS  Google Scholar 

  25. Szymańska E, Winnicka K (2015) Stability of chitosan—a challenge for pharmaceutical and biomedical applications. Mar Drugs 13(4):1819–1846

    Article  Google Scholar 

  26. Dotto GL, Campana-Filho SP, de Almeida Pinto LA (2017) Frontiers in biomaterials: chitosan based materials and its applications, vol 3. Bentham Science Publishers, Sharjah

    Book  Google Scholar 

  27. Chang W, Little J (1991) Delayed reproductive death in X-irradiated Chinese hamster ovary cells. Int J Radiat Biol 60(3):483–496

    Article  CAS  Google Scholar 

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Acknowledgments

The findings presented here are financially supported by Science and Engineering Research Board (SERB) under grant number ECR/2016/001429.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Kashmir, Srinagar, Jammu and Kashmir, India

    Taha Umair Wani & Nisar Ahmad Khan

  2. Department of Nanotechnology, University of Kashmir, Srinagar, Jammu and Kashmir, India

    Hasham S. Sofi & Faheem A. Sheikh

Authors
  1. Taha Umair Wani
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  2. Hasham S. Sofi
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  3. Nisar Ahmad Khan
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  4. Faheem A. Sheikh
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Corresponding author

Correspondence to Faheem A. Sheikh .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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© 2019 Springer Science+Business Media New York

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Wani, T.U., Sofi, H.S., Khan, N.A., Sheikh, F.A. (2019). Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2019_256

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  • DOI: https://doi.org/10.1007/7651_2019_256

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0359-8

  • Online ISBN: 978-1-0716-0360-4

  • eBook Packages: Springer Protocols

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