Abstract
Epidermal stem cells are responsible for normal tissue homeostasis and contribute to tissue regeneration during injury. Several assays measuring stem cell frequency and function can be used to assess epidermal stem cell potential. However, the ultimate assay that accounts for stemness is the capacity to sustain in vivo long-term tissue regeneration and maintenance. We can use this type of analysis to interrogate whether a specific genetic alteration (e.g., activation or inactivation of any gene thought to be involved in stem cell quiescence or proliferation) confers increased or decreased stem cell potential.
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References
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Acknowledgments
Work in our laboratory is supported by FEDER cofounded ISCIII grants [CB16/12/00228 and PI18/00263] and Foundation of Fanconi Anemia grant [2018/127].
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Segrelles, C., Santos-de-Frutos, K., Paramio, J.M., Lorz, C. (2019). Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_234
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DOI: https://doi.org/10.1007/7651_2019_234
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