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Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2109))

Abstract

Cell therapy is one of the most hopeful technologies of regenerative medicine approaches. Among various cells, human skin fibroblasts have been progressively used for wound healing as cell-based therapy purposes. By increasing the age, the number of skin fibroblasts’ abilities including cell migration, growth, collagen production, etc. decreases. Hence, use of the fetal source is more beneficent. In this respect, this chapter covers the manufacturing of human fetal skin-derived fibroblasts for clinical application.

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Abbreviations

bFGF:

Basic fibroblast growth factor

CBMPs:

Cell-based medicinal products

CD:

Cluster of differentiation

cGMP:

Current good manufacturing practice

CPMP:

Committee for Proprietary Medicinal Product

CVMP:

Committee for Medicinal Products for Veterinary Use

DMEM-LG:

Dulbecco’s Modified Eagle’s Medium-low glucose

DMSO:

Dimethyl sulfoxide

EMA:

European Medicines Agency

FBS:

Fetal bovine serum

HBV:

Hepatitis B virus

HCV:

Hepatitis C virus

HEPA:

High efficiency particulate air

HIV:

Human immunodeficiency virus

kGy:

Kilogray

LMP:

Last menstrual period

PBS:

Phosphate-buffered saline

Pen-Strep:

Penicillin-Streptomycin

RPR:

Rapid plasma reagin

RT-PCR :

Reverse transcription polymerase chain reaction

SOP:

Standard operating procedure

TSE:

Transmissible spongiform encephalopathy

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Acknowledgments

The authors would like to thank Dr. Mohsen Khorshidi, Shokouh Salimi, Maryam Afshari, and Ali Valadkhani for their considerable assistance.

Author information

Authors and Affiliations

  1. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran

    Parisa Goodarzi

  2. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Hamid Reza Aghayan, Masoumeh Sarvari, Najmeh Foroughi Heravani, Mahdieh Hadavandkhani & Babak Arjmand

  3. Obesity and Eating Habits Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Moloud Payab

  4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical sciences, Tehran, Iran

    Bagher Larijani

  5. Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Sepideh Alavi-Moghadam & Babak Arjmand

  6. Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Hossein Adibi

  7. Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Fatemeh Khatami

Authors
  1. Parisa Goodarzi
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  2. Hamid Reza Aghayan
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  3. Moloud Payab
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  4. Bagher Larijani
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  5. Sepideh Alavi-Moghadam
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  6. Masoumeh Sarvari
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  7. Hossein Adibi
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  8. Fatemeh Khatami
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  9. Najmeh Foroughi Heravani
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  10. Mahdieh Hadavandkhani
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  11. Babak Arjmand
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Corresponding author

Correspondence to Babak Arjmand .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Goodarzi, P. et al. (2019). Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_233

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  • DOI: https://doi.org/10.1007/7651_2019_233

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0250-8

  • Online ISBN: 978-1-0716-0251-5

  • eBook Packages: Springer Protocols

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