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Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy

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Imaging and Tracking Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2150))

Abstract

Intravital multiphoton microscopy of the kidney is a powerful technique to study alterations in tissue morphology and function simultaneously in the living animal and represents a dynamic and developing research tool in the field. Recent technological advances include serial intravital multiphoton microscopy of the same kidney regions over several weeks and combined with ex vivo histology for cellular biomarker expression of the same cells, which had been subject to serial imaging before. Thus, serial intravital multiphoton microscopy followed by ex vivo histology provides unique tools to perform long-term cell fate tracing of the same renal cells during physiological and pathophysiological conditions, thereby allowing the detection of structural changes of the same renal cells over time. Examples include renal cell migration and proliferation while linking these events to local functional alterations and eventually to the expression of distinct cellular biomarkers. Here, we provide a detailed step-by-step protocol to facilitate serial intravital multiphoton microscopy for long-term in vivo tracking of renal cells and subsequent ex vivo histology for immunohistological staining of the same cells in the fixed tissue.

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Change history

  • 19 February 2020

    The original version of this chapter was inadvertently published without a proper acknowledgement. The authors informed to insert the following acknowledgement in this chapter.

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Acknowledgments

This work was supported in part by US National Institutes of Health grants DK064324, DK100944, and S10OD021833 and by Lupus Research Alliance grant 519100 to J.P-P. IMS was funded by a postdoctoral research fellowship of the German Research Foundation.

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Authors and Affiliations

  1. Department of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Ina Maria Schiessl & Janos Peti-Peterdi

  2. Institute of Physiology, University of Regensburg, Regensburg, Germany

    Katharina Fremter & Hayo Castrop

  3. Department of Ophthalmology, University of Southern California, Los Angeles, CA, USA

    James L. Burford

Authors
  1. Ina Maria Schiessl
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  2. Katharina Fremter
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  3. James L. Burford
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  4. Hayo Castrop
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  5. Janos Peti-Peterdi
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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Schiessl, I.M., Fremter, K., Burford, J.L., Castrop, H., Peti-Peterdi, J. (2019). Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 2150. Humana, New York, NY. https://doi.org/10.1007/7651_2019_232

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  • DOI: https://doi.org/10.1007/7651_2019_232

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0626-1

  • Online ISBN: 978-1-0716-0627-8

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