Abstract
Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which autophagy plays an important role. It has been shown that autophagy deficiency dampens adipocyte differentiation, compromises adipose tissue development, dysregulates adipocytokine secretion, and even causes sudden death in young animals. Therefore, accurate assessment of autophagy in adipocyte is critical for the study of adipose biology or pathology of metabolic diseases. In this chapter, we described the procedure of autophagy analysis during adipocyte differentiation, and discussed the power of steady-state autophagy protein (e.g., beclin 1, LC3, and p62) levels versus autophagy flux to reflect autophagy activity.
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Acknowledgment
This work was supported in part by USDA National Institute of Food and Agriculture Hatch Project 1007334 (ZC).
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Tao, Z., Liu, L., Zheng, L.D., Cheng, Z. (2017). Autophagy in Adipocyte Differentiation. In: Turksen, K. (eds) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, vol 1854. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_65
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DOI: https://doi.org/10.1007/7651_2017_65
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8747-4
Online ISBN: 978-1-4939-8748-1
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