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Organoids pp 23–31Cite as

Organoid Culture of Human Cancer Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.

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Acknowledgments

We thank the divisions of Breast and Endocrine Surgery and of Gastrointestinal Surgery of Kobe University Graduate School of Medicine, and the division of Surgery of Nagoya University for contribution in the patient tumor collection.

This study was supported by Grants-in-Aid for Scientific Research from the Japan Society of the Promotion of Science and by the grants from Japan Foundation for Applied Enzymology and the Itoh-Chubei Foundation to Y.S.

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Authors and Affiliations

  1. Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan

    Yohei Shimono, Junko Mukohyama & Akira Suzuki

  2. Department of Pathology and Cell Biology, Columbia University, New York, NY, USA

    Piero Dalerba

  3. Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA

    Darius M. Johnston

  4. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA

    Taichi Isobe

Authors
  1. Yohei Shimono
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  2. Junko Mukohyama
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  3. Taichi Isobe
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  4. Darius M. Johnston
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  5. Piero Dalerba
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  6. Akira Suzuki
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Corresponding author

Correspondence to Yohei Shimono .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Shimono, Y., Mukohyama, J., Isobe, T., Johnston, D.M., Dalerba, P., Suzuki, A. (2016). Organoid Culture of Human Cancer Stem Cells. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_13

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  • DOI: https://doi.org/10.1007/7651_2016_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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