The Selection of Recombinant Binary Plasmids Generated by Gateway® LR Cloning in the Escherichia coli Strain C2110 Shuchi WuBingyu Zhao Research 04 May 2012 Pages: 125 - 132
An Alternative Approach in Gateway® Cloning when the Bacterial Antibiotic Selection Cassettes of the Entry Clone and Destination Vector are the Same Kamal KumarSaurabh YadavPraveen Kumar Verma Research 04 May 2012 Pages: 133 - 140
A Novel Bifunctional Protein TNFR2-Fc-IL-1ra (TFI): Expression, Purification and its Neutralization Activity of Inflammatory Factors Bo XieShan LiuMizhou Hui Research 08 May 2012 Pages: 141 - 147
The hdhA Gene Encodes a Haloacid Dehalogenase that is Regulated by the LysR-Type Regulator, HdhR, in Sinorhizobium meliloti Ratiboot SallabhanJarunee KerdwongSuvit Loprasert Research 26 May 2012 Pages: 148 - 157
Comparison of the Heterologous Expression of Trichoderma reesei Endoglucanase II and Cellobiohydrolase II in the Yeasts Pichia pastoris and Yarrowia lipolytica Nassapat BoonvitthyaSophie BozonnetWarawut Chulalaksananukul Research 26 May 2012 Pages: 158 - 169
Construction and Application of a Food-Grade Expression System for Lactococcus lactis Wenwei LuJian KongWentao Kong Research 07 June 2012 Pages: 170 - 176
Gene Expression and DNA Methylation Status of Chicken Primordial Germ Cells Hyun-Jun JangHee Won SeoJae Yong Han Research 08 June 2012 Pages: 177 - 186
Parallel Peptidome and Transcriptome Analyses of Amphibian Skin Secretions Using Archived Frozen Acid-Solvated Samples Bing BaiYingqi ZhangChris Shaw Research 10 May 2012 Pages: 187 - 197
Inhibition of Binding of Tomato Yellow Leaf Curl Virus Rep to its Replication Origin by Artificial Zinc-Finger Protein Tomoaki MoriKosuke TakenakaTakashi Sera Research 11 May 2012 Pages: 198 - 203
Sensitivity of Prestaining RNA with Ethidium Bromide Before Electrophoresis and Performance of Subsequent Northern Blots Using Heterologous DNA Probes Yun ZhaoLinfang DuNianhui Zhang Research 15 May 2012 Pages: 204 - 210
Development of a New Method for Genetic Transformation of the Green Alga Chlorella ellipsoidea Lili LiuYanqi WangShengwu Ma Research 13 May 2012 Pages: 211 - 219
Transfection Efficiency Along the Regenerating Soleus Muscle of the Rat Magdolna KósaErnő Zádor Research 12 May 2012 Pages: 220 - 227
Generation and Characterization of Yeast Two-Hybrid cDNA Libraries Derived From Two Distinct Mouse Pluripotent Cell Types Ying ZhengXiaoying TanWolfgang Engel Research Open access 07 June 2012 Pages: 228 - 237
Alternative Promoters Regulate Cold Inducible RNA-Binding (CIRP) Gene Expression and Enhance Transgene Expression in Mammalian Cells Mohamed B. Al-FageehC. Mark Smales OriginalPaper 16 April 2013 Pages: 238 - 249
Increased Understanding of the Biochemistry and Biosynthesis of MUC2 and Other Gel-Forming Mucins Through the Recombinant Expression of Their Protein Domains Malin BäckströmDaniel AmbortGunnar C. Hansson Original Paper 29 January 2013 Pages: 250 - 256
Protein Bodies in Nature and Biotechnology Stefan R. Schmidt Research 29 January 2013 Pages: 257 - 268
Anti-Digoxin Fab Variants Generated by Phage Display Viviane Midori MurataMariana Costa Braga SchmidtAna Maria Moro Original Paper 29 January 2013 Pages: 269 - 277
Validation of Reference Genes for Normalizing Gene Expression in Real-Time Quantitative Reverse Transcription PCR in Human Thyroid Cells in Primary Culture Treated with Progesterone and Estradiol Ana Paula SantinAline Francielle Damo SouzaTania Weber Furlanetto Research 06 June 2012 Pages: 278 - 282
Identification of Protease Inhibitors by a Fast Fluorimetric Assay Nunzianna DotiDomenico RaimondoMenotti Ruvo Research 10 June 2012 Pages: 283 - 291
The Xerophyta viscosa Aldose Reductase (ALDRXV4) Confers Enhanced Drought and Salinity Tolerance to Transgenic Tobacco Plants by Scavenging Methylglyoxal and Reducing the Membrane Damage Deepak KumarPreeti SinghNeera Bhalla Sarin Research 08 June 2012 Pages: 292 - 303
In Vitro Ca2+-Dependent Maturation of Milk-Clotting Recombinant Epr: Minor Extracellular Protease: From Bacillus licheniformis José Manuel AgeitosJuan Andrés VallejoTomás G. Villa Research 27 June 2012 Pages: 304 - 311
Prokaryotic Expression and In Vitro Functional Analysis of IL-1β and MCP-1 from Guinea Pig Vijaya R. DirisalaAmminikutty JeevanDavid N. McMurray Research 29 June 2012 Pages: 312 - 319
The Choline Oxidase Gene codA Confers Salt Tolerance to Transgenic Eucalyptus globulus in a Semi-Confined Condition Xiang YuAkira KikuchiKazuo N. Watanabe Research 03 July 2012 Pages: 320 - 330
The Effect of the Microgravity Rotating Culture System on the Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Xing WuShao-hua LiZheng-rong Chen Research 06 June 2012 Pages: 331 - 336
Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures Ildikó VashegyiZsuzsa Marozsán-TóthBalázs Tóth Research 06 June 2012 Pages: 337 - 349
Targeting of the Enhanced Green Fluorescent Protein Reporter to Adrenergic Cells in Mice Jixiang XiaNamita VarudkarSteven N. Ebert Research 16 June 2012 Pages: 350 - 360
Development of Bead-based Immunoassay to Quantify Neutralizing Antibody for Human Papillomavirus 16 and 18 Hai-Bo LiuPankaj Kumar ChaturvediWoong Shick Ahn Research 06 June 2012 Pages: 361 - 370
Effect of Biofilm Formation by Bacillus subtilis natto on Menaquinone-7 Biosynthesis Aydin BerenjianNatalie Li-Cheng ChanFariba Dehghani Research 28 June 2012 Pages: 371 - 378
Improved Growth, Drought Tolerance, and Ultrastructural Evidence of Increased Turgidity in Tobacco Plants Overexpressing Arabidopsis Vacuolar Pyrophosphatase (AVP1) Anjuman ArifYusuf ZafarEduardo Blumwald Research 11 July 2012 Pages: 379 - 392
Anatomical and Physiological Differences and Differentially Expressed Genes Between the Green and Yellow Leaf Tissue in a Variegated Chrysanthemum Variety Qingshan ChangSumei ChenShuwei Wang Research 11 July 2012 Pages: 393 - 411
Pathogenesis-Related Gene, JcPR-10a from Jatropha curcas Exhibit RNase and Antifungal Activity Parinita AgarwalVacha BhattJitendra Chikara Research 05 July 2012 Pages: 412 - 425
Overexpression of CsANR Increased Flavan-3-ols and Decreased Anthocyanins in Transgenic Tobacco Vinay KumarSudesh Kumar Yadav Research 08 August 2012 Pages: 426 - 435
A Novel Dual Vector Coexpressing PhiX174 Lysis E Gene and Staphylococcal Nuclease A Gene on the Basis of Lambda Promoter pR and pL, Respectively Lixia FuChengping Lu Research 11 July 2012 Pages: 436 - 444
An Epidermal Growth Factor Motif from Del1 Protein Increases the Efficiency of In Vivo Gene Transfer with a Non-Viral Vector Atsushi MamiyaHisataka KitanoChiaki Hidai Research 11 July 2012 Pages: 445 - 450
Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L Maoyun SheGuixiang YinXingguo Ye Research 20 July 2012 Pages: 451 - 460
KIT Proto-oncogene Exon 8 Deletions at Codon 419 are Highly Frequent in Acute Myeloid Leukaemia with Inv(16) in Indian Population Syed Rizwan HussainHena NaqviSunil G. Babu Research 24 July 2012 Pages: 461 - 468
Molecular Cloning, Co-expression, and Characterization of Glycerol Dehydratase and 1,3-Propanediol Dehydrogenase from Citrobacter freundii Xianghui QiWenying DengRibo Huang Research 22 July 2012 Pages: 469 - 474
Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease Alejandra LarsenEster Teresa GonzalezEduardo Mortola Research 25 July 2012 Pages: 475 - 483
Studies on the Refolding Process of Recombinant Horseradish Peroxidase Sedigheh AsadBahareh DabirmaneshKhosro Khajeh Research 08 August 2012 Pages: 484 - 492
A Quantitative Real-Time PCR Method Using an X-Linked Gene for Sex Typing in Pigs Maria BallesterAnna CastellóJosep M. Folch Research 28 July 2012 Pages: 493 - 496
Productivity and Quality of Recombinant Proteins Produced by Stable CHO Cell Clones can be Predicted by Transient Expression in HEK Cells Carolin DiepenbruckMatthias KlingerRüdiger Neef Research 23 August 2012 Pages: 497 - 503
A Low-Copy-Number Plasmid for Retrieval of Toxic Genes from BACs and Generation of Conditional Targeting Constructs Giyoun NaAndrew WolfeYongbum Koo Research 04 September 2012 Pages: 504 - 514
In Vitro Molecular Characterization of RNA–Proteins Interactions During Initiation of Translation of a Wild-Type and a Mutant Coxsackievirus B3 RNAs Amira SouiiManel Ben M’hadheb-GharbiJawhar Gharbi Research 25 August 2012 Pages: 515 - 527
Chloroplast Genome Diversity in Portuguese Grapevine (Vitis vinifera L.) Cultivars Isaura CastroOlinda Pinto-CarnideJuan Pedro Martín Research 24 August 2012 Pages: 528 - 540
A Linear Concatenation Strategy to Construct 5′-Enriched Amplified cDNA Libraries Using Multiple Displacement Amplification Vijay J. GadkarMartin Filion Research 01 September 2012 Pages: 541 - 550
Overexpression of Alternative Oxidase Gene Confers Aluminum Tolerance by Altering the Respiratory Capacity and the Response to Oxidative Stress in Tobacco Cells Sanjib Kumar PandaLingaraj SahooHideaki Matsumoto Research 11 September 2012 Pages: 551 - 563
First Knockdown Gene Expression in Bat (Hipposideros armiger) Brain Mediated by Lentivirus Qi ChenTengteng ZhuYi Sun Research 11 September 2012 Pages: 564 - 571
Site-Directed Mutations of the Gatekeeping Loop Region Affect the Activity of Escherichia coli Spermidine Synthase Mon-Juan LeeYa-Ting YangHaimei Huang Research 23 September 2012 Pages: 572 - 580
Sangiovese and Its Offspring in Southern Italy Marica GasparroAngelo Raffaele CaputoDonato Antonacci Research 22 September 2012 Pages: 581 - 589
Development of Oligoclonal Nanobodies for Targeting the Tumor-Associated Glycoprotein 72 Antigen Zahra SharifzadehFatemeh RahbarizadehSeyed Hamid Aghaee Bakhtiari Research 27 September 2012 Pages: 590 - 601
