1. Establishment of stable melanoma cell line expressing a novel gene, Jpk, using a tetracycline-controlled gene expression system

   • Byung-Gyu Kim
   • Meang Sub Cheng
   • Myoung Hee Kim

   Research Pages: 1 - 6

2. Variations in transfection efficiency of VEGF₁₆₅ and VEGF₁₂₁-cDNA

   • Srdjan Djurovic
   • Heidi Kristansen
   • Kåre Berg

   Research Pages: 7 - 16

3. Expression of modified Cry1Ac gene of Bacillus thuringiensis in transgenic tobacco plants

   • Lucyna Honorata Misztal
   • Adrianna Mostowska
   • Artur Jarmolowski

   Research Pages: 17 - 26

4. A novel simple and rapid PCR-based site-directed mutagenesis method

   • Imen Rabhi
   • Naouel Guedel
   • Dahmani M. Fathallah

   Research Pages: 27 - 34

5. Cloning genes from a library using a clustering strategy and PCR

   • S. Díaz Prado
   • N. Tarrío
   • M. I. González Siso

   Research Protocol Pages: 35 - 38

6. Generation and production of engineered antibodies

   • Sergey M. Kipriyanov
   • Fabrice Le Gall

   Review Pages: 39 - 60

7. Specificity and performance of PCR detection assays for microbial pathogens

   • Konrad Sachse

   Review Pages: 61 - 79

8. Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae

   • Francesco Iannelli
   • Gianni Pozzi

   Protocol Pages: 81 - 86

9. Rapid genotyping of the osteoporosis-associated polymorphic transcription factor Sp1 binding site in the COL1A1 gene by pyrosequencing

   • Katarina Ellnebo-Svedlund
   • Lasse Larsson
   • Per Magnusson

   Hints And Tips Pages: 87 - 90

10. Book reviews

    • Fiona Thompson
    • Matthew Crook
    • Kay Roberts

    Departments Pages: 91 - 91

11. New products for molecular biology

    Departments Pages: 92 - 92

12. Calendar

    Departments Pages: 93 - 93