Abstract
A process for industrial production of fructooligosaccharides (FOS’s) based on the conversion of sucrose by immobilized fructosyltransferase (FTase) from the cells of Aureobasidium pullulans CCY 27-1-94 was developed. Particular process operations and conditions were designed employing results of laboratory and semi-pilot scale experiments. The process flowsheet comprised three sections: FTase production, which included fermentation, isolation and purification of the enzyme, FTase immobilization and FOS’s production where a product with a high content of FOS’s was prepared by the removal of glucose, fructose and unreacted sucrose from the reaction mixture using simulated moving-bed chromatography. Two alternative process flowsheets were proposed for the annual production of 10 000 t of FOS’s: one for a powdery product and the second one for syrup. The economic analysis provided the costs for the production of immobilized FTase and FOS’s using two different price estimates for sucrose.
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References
Aboudzadeh, M. R., Jiawen, Z., & Bin, W. (2006). Modeling of protein adsorption to DEAE Sepharose FF: Comparison of data with model simulation. Korean Journal of Chemical Engineering, 23, 124–130. DOI: 10.1007/BF02705703.
Aydogan, N., Gurkan, T., & Yilmaz, L. (1998). Effect of operating parameters on the separation of sugars by nanofiltration. Separation Science and Technology, 33, 1767–1785.
Bekers, M., Laukevics, J., Upite, D., Kaminska, E., Vigants, A., Viesturs, U., Pankova, L., & Danilevics, A. (2002). Fructooligosaccharide and levan producing activity of Zymomonas mobilis extracellular levansucrase. Process Biochemistry, 38, 701–706. DOI: 10.1016/S0032-9592(02)00189-9.
Crittenden, R. G., & Playne, M. J. (1996). Production, properties and applications of food-grade oligosaccharides. Trends in Food Science & Technology, 7, 353–361. DOI: 10.1016/S0924-2244(96) 10038-8.
Flamm, G., Glinsmann, W., Kritchevsky, D., Prosky, L., & Roberfroid, M. (2001). Inulin and oligofructose as dietary fiber: a review of the evidence. Critical Reviews in Food Science and Nutrition, 41, 353–362. DOI: 10.1080/20014091091841.
Franck, A. (2002). Technological functionality of inulin and oligofructose. British Journal of Nutrition, 87, 287–291. DOI: 10.1079/BJN/2002550.
Garleb, K. A., Snook, J. T., Marcon, M. J., Wolf, B. W., & Johnson, W. A. (1996). Effect of fructooligosaccharide containing enteral formulas on subjective tolerance factors, serum chemistry profiles, and feacal bifidobacteria in healthy adult male subjects. Microbial Ecology in Health and Disease, 9, 279–285.
Ghazi, I., Fernandez-Arrojo, L., Garcia-Arellano, H., Ferrer, M., Ballesteros, A., & Plou, F. J. (2007). Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus. Journal of Biotechnology, 128, 204–211. DOI: 10.1016/j.jbiotec.2006.09.017.
Goulas, A. K., Kapasakalidis, P. G., Sinclair, H. R., Rastall, R. A., & Grandison, A. S. (2002). Purification of oligosaccha-rides by nanofiltration. Journal of Membrane Science, 209, 321–335. DOI: 10.1016/S0376-7388(02)00362-9.
Gramblička, M., & Polakovič, M. (2007). Adsorption equilibria of glucose, fructose, sucrose, and fructooligosaccharides on cation exchange resin. Journal of Chemical & Engineering Data, 52, 345–350. DOI: 10.1021/je060169d.
Heinzle, E., Biwer, A. P., & Cooney, C. L. (2006). Development of sustainable bioprocesses. Hoboken: John Wiley & Sons.
Charton, F., & Nicoud, R.-M. (1995). Complete design of a simulated moving bed. Journal of Chromatography A, 702, 97–112. DOI: 10.1016/0021-9673(94)01026-B.
Chen, W. C., & Liu, C. H. (1996). Production of beta-fructofuranosidase by Aspergillus japonicus. Enzyme and Microbial Technology, 18, 153–160. DOI: 10.1016/0141-0229(95)00099-2.
Chien, C.-S., Lee, W.-C., & Lin, T.-J. (2001). Immobilization of Aspergillus japonicus by entrapping cells in gluten for production of fructooligosaccharides. Enzyme and Microbial Technology, 29, 252–257. DOI: 10.1016/S0141-0229(01)00384-2.
Jung, K. H., Yun, J. W., Kang, K. R., Lim, J. Y., & Lee, J. H. (1989). Mathematical model for enzymatic production of fructo-oligosaccharides from sucrose. Enzyme and Microbial Technology, 11, 491–494. DOI: 10.1016/0141-0229(89)90029-X.
Kim, B. W., Kishihara, S., & Satoshi, F. (1992). Simultaneously continuous separation of glucose,maltose, and maltotriose using a simulated moving-bed adsorbent. Bioscience, Biotechnology, and Biochemistry, 56, 801–802.
L’Hocine, L., Wang, Z., Jiang, B., & Xu, S. (2000). Purification and partial characterization of fructosyltransferase and invertase from Aspergillus niger AS0023. Journal of Biotechnology, 81, 73–84. DOI: 10.1016/S0168-1656(00)00277-7.
L’Homme, C., Puigserver, A., & Biagini, A. (2003). Effect of food-processing on the degradation of fructooligosaccharides in fruit. Food Chemistry, 82, 533–537. DOI:10.1016/S0308-8146(03)00003-7.
Lee, K. J., Choi, J. D., & Lim, J. Y. (1992). Purification and properties of intracellular fructosyl transferase from Aureobasidium pullulans. World Journal of Microbiology & Biotechnology, 8, 411–415.
Madlová, A., Antošová, M., Baráthová, M., Polakovič, M., Štefuca, V., & Báleš, V. (1999). Screening of microorganisms for transfructosylating activity and optimization of biotransformation of sucrose to fructooligosaccharrides. Chemical Papers, 53, 366–369.
Nguyen, Q. D., Rezessy-Szabo, J. M., Bhat, M. K., & Hoschke, A., (2005). Purification and some properties of [α]-fructofuranosidase from Aspergillus niger IMI303386. Process Biochemistry, 40, 2461–2466. DOI: 10.1016/j.procbio. 2004.09.012.
Nizhizawa, K., Nakajima, M., & Nabetani, H. (2001). Kinetic study on transfructosylation by fructofuranosidase from Aspergillus niger ATCC 20611 and availability of a membranereactor for fructooligosaccharide production. Food Science and Technology Research, 7, 39–44.
Onderková, Z., Polakovič, M., Štefuca, V., Vandákova, M., & Antošová, M. (2006). Selection of carrier for immobilization of fructosyltransferase from Aureobasidium pullulans. Chemical Papers, 60, 469–472. DOI: 10.2478/s11696-006-0085-x.
Rivero-Urgell, M., & Santamaria-Orleans, A. (2001). Oligosac-charides: application in infant food. Early Human Development, 65(Supplement 2), 43–52. DOI: 10.1016/S0378-3782(01)00202-X.
Sangeetha, P. T., Ramesh, M. N., & Prapulla, S. G. (2004). Production of fructo-oligosaccharides by fructosyltransferase from Aspergillus oryzea CFR 202 and Aureobasidium pullulans CFR 77. Process Biochemistry, 39, 755–760. DOI: 10.1016/S0032-9592(03)00186-9.
Sangeetha, P. T., Ramesh, M. N., & Prapulla, S. G. (2005a). Recent trends in the microbial production, analysis and application of fructooligosaccharides. Trends in Food Science & Technology, 16, 442–457. DOI: 10.1016/j.tifs.2005.05.003.
Sangeetha, P. T., Ramesh, M. N., & Prapulla, S. G. (2005b). Maximization of fructooligosaccharide production by two stage continuous process and its scale up. Journal of Food Engineering, 68, 57–64. DOI: 10.1016/j.jfoodeng.2004.05.022.
Sangeetha, P. T., Ramesh, M. N., & Prapulla, S. G. (2005c). Fructooligosaccharide production using fructosyl transferase obtained from recycling culture of Aspergillus oryzae CFR 202. Process Biochemistry, 40, 1085–1088. DOI: 10.1016/j. procbio.2004.03.009.
Spiegel, J. E., Rose, R., Karabell, P. Frankos, V. H., & Schmitt, D. F. (1994). Safety and benefits of fructooligosaccharides as food ingredients. Food Technology, 48, 85–89.
Takahashi, Y., & Goto, S. (1994). Continuous separation of fructooligosaccharides using an annular chromatograph. Separation Science and Technology, 29, 1311–1318. DOI: 10.1080/01496399408006942.
Tanriseven, A., & Aslan, Y. (2005). Immobilization of Pectinex Ultra SP-L to produce fructooligosaccharides. Enzyme and Microbial Technology, 36, 550–554. DOI: 10.1016/j.enzmictec. 2004.12.001.
Vandáková, M., Platková, Z., Antošová, M., Báleš, V., & Polakovič, M., (2004). Optimization of cultivation conditions for production of fructosyltransferase by Aureobasidium pullulans. Chemical Papers, 58, 15–22.
Vandáková, M., Vaňková, K., Juraščík, M., Annus, J., Minárik, M., & Polakovič, M. (2007). Fructosyltransferase production and isolation in semi-pilot scale. In Proceedings of the 34th International Conference of the Slovak Society of Chemical Engineering, 21–25 May 2007. Tatranské Matliare, Slovakia: Slovak Society of Chemical Engineering.
Vaňková, K., Antošová, M., & Polakovič, M., (2005). Design and economics of industrial production of fructosyltransferase. Chemical Papers, 59, 441–448.
Vente, J. A. (2005). Adsorbent functionality in relation to selectivity and capacity in oligosaccharide separations. PhD. Thesis, University of Twente, the Netherlands.
Yun, J. W. (1996). Fructooligosaccharides-Occurrence, preparation, and application. Enzyme and Microbial Technology, 19, 107–117. DOI: 10.1016/0141-0229(95)00188-3.
Yun, J. W., Kim, D. H., Kim, B. W., & Song, S. K. (1997). Comparison of sugar compositions between inulo-and fructo-oligosacharides produced by different enzymes forms. Biotechnology Letters, 19, 553–556. DOI: 10.1023/A: 1018393505192.
Yun J. W., Lee, M., G., & Song, S. K., (1994). Batch production of high-content fruto-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase. Journal of Fermentation and Bio engineering, 77, 159–163. DOI: 10.1016/0922-338X(94)90316-6.
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Vaňková, K., Onderková, Z., Antošová, M. et al. Design and economics of industrial production of fructooligosaccharides. Chem. Pap. 62, 375–381 (2008). https://doi.org/10.2478/s11696-008-0034-y
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DOI: https://doi.org/10.2478/s11696-008-0034-y