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Baculovirus Expression Protocols

  • Book
  • © 1995

Overview

Editors:
  1. Christopher D. Richardson

    1. Amgen and Ontario Cancer Institutes, Toronto, Canada
      Department of Medical Biophysics, University of Toronto, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 39)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction to the Molecular Biology of Baculoviruses

    • Jorge E. Vialard, Basil M. Arif, Christopher D. Richardson
    Pages 1-24

  3. Baculovirus Transfer Vectors Miguel LÓpez- Ferber

    • William P. Sisk, Robert D. Possee
    Pages 25-63

  4. Insect Cell- Culture Techniques in Serum- Containing Medium

    • Stefan A. Weiss, William G. Whitford, Stephen F. Gorfien, Glenn P. Godwin
    Pages 65-78

  5. Insect Cell Culture in Serum- Free Media

    • Stefan A Weiss, Glenn P. Godwin, Stephen F Gorfien, William G. Whitford
    Pages 79-95

  6. Transfection Techniques for Producing Recombinant Baculoviruses

    • Karen Munkenbeck Trotter, H. Alan Wood
    Pages 97-105

  7. Selection of Recombinant Baculoviruses by Visual Screening

    • Martin J. Page, Brian C. Rodgers
    Pages 107-127

  8. Production of recombinant baculoviruses using linearized viral DNA

    • Paul A Kitts
    Pages 129-142

  9. Rapid procedures for the isolation and PCR analysis of recombinant baculovirus

    • Adrienne Day, Tamara Wright, Arnold Sewall, Marjorie Price-Laface, Niharika Srivastava, Malcolm Finlayson
    Pages 143-159

  10. Production of recombinant baculoviruses using rapid screening vectors that contain the gene for ß -Galactosidase

    • Manon Lalumière, Christopher D. Richardson
    Pages 161-177

  11. Fast isolation of recombinant baculovirus by antibody screening

    • Frank Grosse, Andreas Manns
    Pages 179-185

  12. Continuous foreign gene expression in transformed lepidopteran insect cells

    • Donald L. Jarvis, Linda A. Guarino
    Pages 187-202

  13. Scale-Up of recombinant virus and protein production in Stirred-Tank reactors

    • Rosanne L. Tom, Antoine W. Caron, Bernard Massie, Amine A Kamen
    Pages 203-224

  14. Expression of Tissue Inhibitor of Metalloproteinases by Recombinant Baculovirus-Infected Insect Cells Cultured in an Airlift Fermentor

    • Laurie K Overton, Indravadan Patel, J. David Becherer, Gyan Chandra, Thomas A. Kost
    Pages 225-242

  15. Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors

    • Prabhakara V Choudary, Shizuo G. Kamita, Susumu Maeda
    Pages 243-264

  16. Expression of Foreign Proteins in Trichoplusia ni Larvae

    • Jeffrey A. Medin, Karen Gathy, Mary Sue Coleman
    Pages 265-275

  17. Use of Baculoviruses as Biological Insecticides

    • Jenny S. Gory, David H. L. Bishop
    Pages 277-294

  18. Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors

    • C. Yong Kang
    Pages 295-315

  19. Expression of the Hemagglutinin Protein of Influenza Virus

    • Evelyne Kretzschmar, Kazumichi Kuroda, Hans-Dieter Klenk
    Pages 317-336

  20. Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose

    • Eric J. Sorscher, Maja A. Sommerfelt
    Pages 337-348
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Reviews

...a successful laboratory manual aimed practical bench scientists interested in making a start at using the baculovirus system.-Molecular Biotechnology

Editors and Affiliations

  • Amgen and Ontario Cancer Institutes, Toronto, Canada

    Christopher D. Richardson

  • Department of Medical Biophysics, University of Toronto, Canada

    Christopher D. Richardson

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