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YAC Protocols pp 27–32Cite as

Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 349))

Abstract

Large-scale genome mapping and sequencing projects nowadays rely on robotic systems for yeast artificial chromosome (YAC) library processing, handling, and analysis. The advantages are obvious. Robots are fast, less labor intense, and the data reproducibility is high. However, there may be cases in which automated systems are not available or are unnecessary, for example, if the YAC library is small. In this chapter, we describe a simple method to process YAC libraries manually and screen them by conventional filter hybridization.

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References

  1. Olsen, A. S., Combs, J., Garcia, E., et al. (1993) Automated production of high density cosmid and YAC colony filters using a robotic workstation. Biotechniques 14, 116–123.

    CAS  PubMed  Google Scholar 

  2. Copeland, A. and Lennon, G. (1994) Rapid arrayed filter production using the ‘ORCA’ robot. Nature 369, 421–422.

    Article  CAS  PubMed  Google Scholar 

  3. Brownstein, B. H., Silverman, G. A., Little, R. D., et al. (1989) Isolation of single-copy human genes from a library of yeast artificial chromosome clones. Science 244, 1348–1351.

    Article  CAS  PubMed  Google Scholar 

  4. Green, E. D. and Olson, M. V. (1990) Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87, 1213–1217.

    Article  CAS  PubMed  Google Scholar 

  5. Ross, M. T., Hoheisel, J. D., Monaco, A. P., Larin, Z., Zehetner, G., and Lehrach, H. (1992) High density gridded YAC filters: their potential as genome mapping tools, in Techniques for the Analysis of Complex Genomes (Anand, R., ed.), Academic Press Ltd., London, pp. 137–153.

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Author information

Authors and Affiliations

  1. Abteilung Parasitologie, Hygiene Institut, Universität Heidelberg, Heidelberg, Germany

    Cecilia P. Sanchez

  2. Department of Parasitology, University of Heidelberg Medical School, Heidelberg, Germany

    Michael Lanzer

Authors
  1. Cecilia P. Sanchez
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  2. Michael Lanzer
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Editor information

Editors and Affiliations

  1. School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, Scotland

    Alasdair MacKenzie

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© 2006 Humana Press Inc., Totowa NJ

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Cite this protocol

Sanchez, C.P., Lanzer, M. (2006). Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization. In: MacKenzie, A. (eds) YAC Protocols. Methods in Molecular Biology™, vol 349. Humana Press. https://doi.org/10.1385/1-59745-158-4:27

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  • DOI: https://doi.org/10.1385/1-59745-158-4:27

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-612-2

  • Online ISBN: 978-1-59745-158-1

  • eBook Packages: Springer Protocols

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